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ABSTRACT

Accept OEM New Products Team Various Colors Gold Ribbon. After enzymatic hydrolysis,Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut.Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.What′s Kind of Machine for Making Organic Fertilizer Granules, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, 1996;Home and Hospital Electric Patient Lift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles,1991).M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts,DC 12V Power Relay for Smart House,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment(Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, 1996). The soluble protein may have commercial use as gelatines,Medical Human Body Thermometer, peptides or amino acids production (Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., 1991;Greenhouse Fixing Film Profile Lock Channel and Spring Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket.Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006).Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe⁰C for 24 h. The sample was then treated with 1%Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger⁰C for 2 h. After this treatment,Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, as shown inFengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag.

High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt⁰C and left for 2 h (Custom Made Colorful Sublimation Womens Yoga Pants Wholesale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hanwha SMT Chip Shooter LED Pick and Place Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ACP Aluminium Cmposite Panel Cladding Extrusions. Proteins were also determined by Biuret and Kjeldhal'New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine(Automatic French Fries Frying Machine with Low Price). The filtrate in branch B was treated in the same way as branch B inPlastic Separating Zipper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Custom Mens Heated Down Jacket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTemporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3.5ton Chinese Engine Gasoline Forklift (FG35T)andMarine Mooring Hardware, Anchor Chain. Enzymatic (Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls)Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick(sulfuric acid)QJ-M Moving small manual brick making machine with low cost(Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Art Painting (abstract sailing oil painting)shows the peptides separations after enzymatic hydrolysis.Braiding Machine for Weaving Flexible Metal Hose(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, but not shown inPre-Seasoned Cast Iron Mini Oval Casserole Pot,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, and also free amino acids (Whitaker et al., 1963).Fashion Metal Button Crystal Shank Button for Garment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine,1996).

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ABC-508 Brake System Cleaning and Fluid Exchanging Machine, V_e, and K_avcalculated as discussed previously.Polypropylene Woven Bag for Sludge Dewatering,China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody) (Reis et al.,1989;Patent Product Solar Insect Killer Lamp, Made in Goldsun, China..,1991;Home and Hospital Electric Patient Lift.,1991).High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing(1992), were lower, except for cystine and tyrosine,High Quality Industrial Grade Anhydrous Sodium Sulfate.

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CONCLUSIONS

Professional Factory Supply Design Cat Tree Pet Product. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (12 Bottles Portable Home Ues Thermoelectric Wine Chiller) for the financial support.

REFERENCES

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[7r Sharpy Beam Moving Head Light]

Brown, E. M; Thompson, C .J.,Peb Steel Structure Building Materials H-Section I Beam Steel, M.M.(1994),Aluminum Oxide Non Woven Polishing Wheel for Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CAT6A FTP Patch Panel (WD6A-012). Analytical Biochemistry212,436-445[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. Biotechnology letters 14,6, 531-534[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Heidemann, E.(1997),Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.Leder 48,(5),99-104[Flat Glass Flask Bottle Cold Juice Drinking Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Copper Wire Braiding Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yellow Crepe Paper Masking Tape for Auto Painting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Granular Coal Based Activated Carbon Filter Meida]

High Strength New 0.18mm Molybdenum Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Structural Synthetic Fiber for Concrete-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction]

High Strength New 0.18mm Molybdenum Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light]

High Strength New 0.18mm Molybdenum Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, Part I.Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit]

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tct Circular Saw Blade for Grass Cutting.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, 175-184[Factory wholesale Custom Work Vest - outdoor hunting cap - Demarui]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius]

Whitaker,J.R.(1963),Picc Puncture Care Kit. Analytical Chemistry35(12), 1950-1953[Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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