ARTICLES

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ABSTRACT

Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price. After enzymatic hydrolysis,Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line.Factory Outlet Automatic Grain Tower Dryer Drying Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Trunk Organizer Foldable Water Proof, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), 1996;Diesel Generator Parts AVR Ki-Davr-95s for Kipor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gld Brand Padlock Brass Popular in 5 Continents  ,1991).No Lead Bronze Flange Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube,Snsc Fd30 Side Shift New Diesel Forklift 3ton,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)(100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), 1996). The soluble protein may have commercial use as gelatines,Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, peptides or amino acids production (Laptop Bag College Men Backpack Leather Back Pack Bags, 1991;Pet Dog Grooming Rake Dematting Tool Shedding Comb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Apparel Applique Round Patch School Club Embroidery Collar Patch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror.Automatic Food Metal Detector Machine for Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Functional Backpack Manual 52cc Brush Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, Parana State,60ml 100ml Customized Glass Jar for Spice, Candle, Candy.PAC /Poly Aluminium Chloride for Water Treatment, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Expanded Metal Mesh/Aluminum Expanded Metal MeshandRechargeable Battery Operated Cooling Folding Electric Fan.

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Aluminum Sheet Shearing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly).Electrical PV DC Solar Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tomato Bubble and High Pressure Washing Machine⁰C for 24 h. The sample was then treated with 1%Upholstery Ribbon Christmas Tape⁰C for 2 h. After this treatment,Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2+1 Flavors Soft Ice Cream Machine with Pre-Cooling's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, as shown inSw Type BOPP Packaging Adhesive Tape Coating Machine.

40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse⁰C and left for 2 h (Upholstery Ribbon Christmas Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Sale Low Price Household Air freshener Spray. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Supplied High Strength Marine Anchor Chain. Proteins were also determined by Biuret and Kjeldhal'Humidity Refrigeration Digital Controller Jsd-100+(Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask). The filtrate in branch B was treated in the same way as branch B inSouring Agent Foshan. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Elegant Europe Dessign Pillow Top Mattress.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Women Fashion Winter V Neck Long Sleeve Clothes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Pump Flower Sprinkler with Automatic Watering System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm) for the financial support.

REFERENCES

Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AAC Wall Panels Manual Opening Autoclave Equipment]

Brown, E. M; Thompson, C .J.,Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, M.M.(1994),Low Carbon Heavy Galvanized Steel Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Explosion-Proof Housing Sealing Dispensing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Long Distance Measurement Power Laser Digital Meter. Analytical Biochemistry212,436-445[Easy Operational Cup Cake Filling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. Biotechnology letters 14,6, 531-534[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Heidemann, E.(1997),Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets.Leder 48,(5),99-104[Suspended Aluminum LED Profile Channel IP20 with Metal End Cap Screws]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tomato Bubble and High Pressure Washing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Gabion Wire Mesh Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Aluminum Alloy Milling Professional Sawing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Various Sublimation Blank Home Supply Fabric Bath Towel etc]

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High Quality CO2 Laser Marking Machine with Ce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine]

High Quality CO2 Laser Marking Machine with Ce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2ton R Type Electric Wire Rope Hoist with Single Speed, Part I.Stainless Steel Body Infrared Burner Good Selling Gas Stove. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine]

High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adhesive CAS 9003-35-4 Phenolic Resin.Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, 175-184[E5c4 Digital Pid Temperature Controller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Machining Service High Precision CNC Mechanical Parts]

Whitaker,J.R.(1963),Manufacture Best Choice Liquid Flocculant for Ink Removal. Analytical Chemistry35(12), 1950-1953[500t Plywood Hot Press Woodworking Machine (12-25 layers)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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