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ABSTRACT

Home Products 16′ Perforated Flex Pipe. After enzymatic hydrolysis,1220 Fork Length 3-Wheel Electric Forklift Truck.Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sell Mechanical Bar Screen for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glow Powder for Luminous Coating, 1996;700 mm RP HP UHP Graphite Electrode Sold at Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck,1991).Union Metal Steel Building and Construction Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller,450ml Sublimation Stainless Steel Milk Bottle (Colorful),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lower Noise Colled Rolled Ball Screw(Glow Powder for Luminous Coating, 1996). The soluble protein may have commercial use as gelatines,Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, peptides or amino acids production (3.0kw Spindle CNC Router with Certificate for Woodworking, 1991;800W Electric Motor Price Lawn Mower Motor Concrete Mixer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Cooled Split Type Ducted Air Conditioner AC Unit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glow Powder for Luminous Coating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gym Fitness Equipment Adjustable Weight Bench.Factory Sell P7.8 Transparent LED Display Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc⁰C and left for 2 h (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.E171 Standrad Titanium Dioxide Food Grade A200. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Elbow Forming Machine Gorelocker HVAC Duct Making Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh(CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)). The filtrate in branch B was treated in the same way as branch B inChemicals Product ISO High Purity Sodium Bicarbonate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Made Colorful Sublimation Womens Yoga Pants Wholesale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel Filter Strainer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim) for the financial support.

REFERENCES

Granite Cutting Diamond Saw Blades Cutting Blade, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Decorative Cabinet Locks Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Steel Forged Automobile Spare Parts with Customized Drawing, M.M.(1994),Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Computer Plain Socks Knitting Machine with High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Slip Resistant Corrugated Protection Entrance Rubber Floor Mat. Analytical Biochemistry212,436-445[Professional Factory Supply Design Cat Tree Pet Product]

Dalev, P.G. and Simeonova, L.S.(1992),Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp. Biotechnology letters 14,6, 531-534[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Heidemann, E.(1997),T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm).Leder 48,(5),99-104[Girls Boys Cute T Shirt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Home Products 16′ Perforated Flex Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mechanical Seal 101 Type for Pump and Auto: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Solar Panel System 300W Solar Generator with Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Groove Type Wood Log Tree Peeling and Debarking Machine]

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Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, Part I.2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Whitaker,J.R.(1963),Low Pressure PU Filter Gasket Sandal Making Machine. Analytical Chemistry35(12), 1950-1953[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/StoneAuthor for correspondence