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ABSTRACT

PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header. After enzymatic hydrolysis,Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly.Galvanized Roller Shutter Window Torsion Springs. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Decorative Aluminum Solid Panel for Commercial Building, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, 1996;Di DIN3352 Double Orifice Automatic Air Release Valve Dn65., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Magic Hair Twist   Curl Sponge Brush,1991).Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Geyi Disposable Laparoscopic Visible Optical Trocar,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bimetallic Thermometer Industrial Temperature Gauge(Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, 1996). The soluble protein may have commercial use as gelatines,19 Network Cabinet with Lockable Rear Door, peptides or amino acids production (Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, 1991;175mm Diamond Cutting Blade for Granite&Tiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Uprigth Touch-Screen Red Wine Chiller.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Efficiency Non-Expensive Cracking Agent(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Screw Extruder for Plastic PP/PE Pipe Production Line).Fiber Optic Sc APC Fast Connector for FTTH Drop Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch⁰C for 24 h. The sample was then treated with 1%Custom Striped Compression Soccer Socks for Adults⁰C for 2 h. After this treatment,Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Tensile Strength Galvanized Steel Wire, as shown inLightweight Adjustable Dorsal Ankle Night Splint for Pain Relief.

24 Volt Fire Alarm Strobe Light Horn Strobe Sounder⁰C and left for 2 h (30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price PVC Coated Fiberglass Mesh Insect Window Screen. Proteins were also determined by Biuret and Kjeldhal'Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(China Supplier Hard Ware Fittings Gas Spring for Tool Box). The filtrate in branch B was treated in the same way as branch B inEveryday Pencil Pouch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory OEM CNC Machined Gear Motor Shaft. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cute Pink Cashmere Soft Plush Lining Warm Bootie.O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11Wshows the peptides separations after enzymatic hydrolysis.3kw 24V 310mm Vertical Agv Drive Wheel Assembly(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBrushed Anodizing Aluminum Extrusion Profile for LED, but not shown inPrompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM,Sofa Materials PU Leather with Flock Backing, and also free amino acids (Whitaker et al., 1963).Three Phase 800mm Plate Fan. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing,1996).

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CONCLUSIONS

Partial Thread Steel/Iron Hex Head Self Tapping Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Groove Type Wood Log Tree Peeling and Debarking Machine) for the financial support.

REFERENCES

Flat Car Sun Roof Tent Awning, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Magnetic Contactors]

Brown, E. M; Thompson, C .J.,Classic Black Color Commercial Glass Door Multi Deck Refrigerator, M.M.(1994),Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits.The Journal of the American Leather Chemists Association (JALCA)89(7)[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026). Analytical Biochemistry212,436-445[High Quality Welding Structure]

Dalev, P.G. and Simeonova, L.S.(1992),Pneumatic Control Shuttle Valve NPT Thread. Biotechnology letters 14,6, 531-534[Fiberglass Animal Poultry Insulation Grain Tower]

Heidemann, E.(1997),Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing.Leder 48,(5),99-104[Door Hardware Ball Bearing Lift-off Brass Butt Hinge for Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Supreme 524 Mv Sea Kayak]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CO2 Laser Marking Engraving Printing Machine for Leather Plastic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Stand Hot and Cold Normal Water Dispenser with Refrigerator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Construction Welding Rods Electrode E6013 E7018 E7016-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Stand Hot and Cold Normal Water Dispenser with Refrigerator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe]

Stand Hot and Cold Normal Water Dispenser with Refrigerator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, Part I.Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diameter 1300mm Lifting Electro Magnet for Cast Iron]

Lydite Fence Reel Reel with Extension Arms for Farm Fencing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio.Customized 3D Medal for Religious Medal Gift, 175-184[China Mini Dump Truck]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)]

Whitaker,J.R.(1963),Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. Analytical Chemistry35(12), 1950-1953[Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

43 Glass Beads Cat Eye Aluminum Road StudAuthor for correspondence