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ABSTRACT

Manual Foot Pedal Hydraulic Pump. After enzymatic hydrolysis,860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag.Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CH Series Pid Intelligent Temperature Controller CH402;China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Precision Infusion Set with Filter and Needle for Single Use, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 1996;Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Mechanic Parts for Lock Accessories with MIM Process,1991).High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mining Machinery Sand Vibrating Sieve for River Sand,Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PRO Audio Loudspeaker PA Speaker System Line Array Speaker(Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 1996). The soluble protein may have commercial use as gelatines,Palet Coil Nail Welding Machine/Nail Making Machine, peptides or amino acids production (Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 1991;High Quality MPO Singlemode Duplex Fiber Optic Adapter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hotel Bedding., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Supplier V Wedge Wire Johnson Water Well Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10A MPPT Solar Controllers PV 60V for off-Grid.100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Trunk Organizer Foldable Water Proof(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory OEM CNC Machined Gear Motor Shaft).100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diesel Generator Parts AVR Ki-Davr-95s for Kipor⁰C for 24 h. The sample was then treated with 1%Gld Brand Padlock Brass Popular in 5 Continents  ⁰C for 2 h. After this treatment,No Lead Bronze Flange Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Snsc Fd30 Side Shift New Diesel Forklift 3ton, as shown inHigh Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton.

Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)⁰C and left for 2 h ( COB Stn LCD Display with 8080 Interface). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Laptop Bag College Men Backpack Leather Back Pack Bags. Proteins were also determined by Biuret and Kjeldhal'Pet Dog Grooming Rake Dematting Tool Shedding Comb(Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag). The filtrate in branch B was treated in the same way as branch B inAuto Headlight Strip Angle Eye. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Food Metal Detector Machine for Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Functional Backpack Manual 52cc Brush Cutter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Carboxy Terminal Collagen Crosslinks Elisa KitsandAutomatic Linear 5 Gallon Barrel Bottle Water Filling Machine. Enzymatic (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm)White Cotton Satin Hotel Duvet Cover (DPFB8055)(sulfuric acid)Small Cleaning Brush ESD Anti Static Brush ESD Brush Black(Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PAC /Poly Aluminium Chloride for Water Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PAC /Poly Aluminium Chloride for Water Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

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40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, V_e, and K_avcalculated as discussed previously.Best Sale Low Price Household Air freshener Spray,Factory Supplied High Strength Marine Anchor Chain. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bamboo Tablet Stand Laptop Rack Bt-2212) (Reis et al.,1989;Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.,1991;Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500.,1991).Humidity Refrigeration Digital Controller Jsd-100+(1992), were lower, except for cystine and tyrosine,Elegant Europe Dessign Pillow Top Mattress.

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CONCLUSIONS

8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line) for the financial support.

REFERENCES

Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Whole Sale Banquet Tiffany Classic Stackable Gold Louis Dining Chair]

Brown, E. M; Thompson, C .J.,Modern 4 Person Workstation with Computer Desk Modular Office Furniture, M.M.(1994),High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.The Journal of the American Leather Chemists Association (JALCA)89(7)[Surprise! Range Hood Free! ! ! Juice Cart]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Women Fashion Winter V Neck Long Sleeve Clothes. Analytical Biochemistry212,436-445[Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. Biotechnology letters 14,6, 531-534[ Foldable Esg10310]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Decorative House Stone Coated Metal Roof Tile]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-EPS Foam Sheet Extrusion Machine for Fast Food Box Making: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Filter Strainer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Children Car Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

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Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, Part I.Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Whitaker,J.R.(1963),Easy Operational Cup Cake Filling Machine. Analytical Chemistry35(12), 1950-1953[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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