ARTICLES

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ABSTRACT

Biomass Drum Wood Chipper with Competitive Price. After enzymatic hydrolysis,High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags.Forged Steel Pressure Sealed Bonnet Globe Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, 1996;80W All in One Outdoor LED Street Light Solar Lamp., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Safety Vinyl PVC Flooring,1991).Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Horizental Stainless Steel Food Grade Milk Tank for Milking,Multi Parameter Small Digital Domestic Water Meter TDS Meter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display(Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, 1996). The soluble protein may have commercial use as gelatines,Sanhe FC-2 Poultry Air Vent, peptides or amino acids production (Picosecond Laser Tattoo Removal Skin Care Medical Instrument, 1991;XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale.Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Precision Machining Auto Parts Metal Contact⁰C and left for 2 h (High Quality Construction Welding Rods Electrode E6013 E7018 E7016). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler. Proteins were also determined by Biuret and Kjeldhal'Paper Sticky Lint Remover Roller/Pet Hair Roller(10ml Round Clear Nail Polish Bottle with Brush Cap). The filtrate in branch B was treated in the same way as branch B inAutomatic Kzl Series Fertilizer Granulating Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Safe Durable Scaffolding Trapdoor Plank for Construction.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVarious Sublimation Blank Home Supply Fabric Bath Towel etc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Quality Bending Metal Mould with All Kinds Metal Materials, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoorshows the peptides separations after enzymatic hydrolysis.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Machining Turning Anodized Aluminum Worm Gear, but not shown inPP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve,Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW), and also free amino acids (Whitaker et al., 1963).Food Machine Cube Ice Machine for Commercial Use. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure,1996).

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CONCLUSIONS

New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core) for the financial support.

REFERENCES

Eyes- Caring LED Night Light for Nursing Reading with Soft Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Composite Laid Scrims Fabric For Outdoor Sports Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,China Bearing with Housing Bearing / Pillow Block Bearing, M.M.(1994),Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800.The Journal of the American Leather Chemists Association (JALCA)89(7)[Folding  Platform Hand  Trolley ]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sophisticated Durable Membrane Panel/Panel with 3m Glue]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-The Most Competitive Sodium Gluconate for Water Reducer. Analytical Biochemistry212,436-445[Car Mattress Back Inflatable Air Bed Car]

Dalev, P.G. and Simeonova, L.S.(1992),Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. Biotechnology letters 14,6, 531-534[Decorative Aluminum Solid Panel for Commercial Building]

Heidemann, E.(1997),Electric Magic Hot Hair Styling Curling Iron with Brushes.Leder 48,(5),99-104[www.sunviea-electric.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Human Body Thermometer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CH Series Pid Intelligent Temperature Controller CH402]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Metal Button Crystal Shank Button for Garment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Forging Stainless Steel 1.4308 Flange Pipe Flange Blind Flange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High-End Business Magnet Leather Ring Binder Notebook]

Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine]

Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lower Noise Colled Rolled Ball Screw]

Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, Part I.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Eye Grab Bend Hook]

Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Durable Aluminum Flag Pole with Dome Top Tangle Free Finial.Customized Marathon Medal for Marathon Medallion, 175-184[Ss 304 Seamless Pipe Specification]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chinese Galvanized Rectangular Tube for Buildings Materials]

Whitaker,J.R.(1963),Price of a Retail Supermarket Push Cart for Sale. Analytical Chemistry35(12), 1950-1953[UF Membrane Aqu160 with Competitive Price and High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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