ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Evaporator Finned Tube. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear

ABSTRACT

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Key words;CNC Turning Center, CNC Horizontal Turning Milling Machine;Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, 1996;Vitamin D3 Fat-Solubility Water-Solubility Feed Additive., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Wholesale Zinc Plated Carbon Steel Hex Nut,1991).Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V,Stainless Steel Cheese Making Machine Electric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts(Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, 1996). The soluble protein may have commercial use as gelatines,100W Dimmable LED Corn Bulb with Meanwell Driver, peptides or amino acids production (Pet and Animal Food Extrusion Machine, 1991;Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hexagonal Wire Mesh Chicken Wire Mesh High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.V-32 New Special UPS Solar Battery Cabinet Box Case.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4-14 Manual Staple Gun with Staples for Hand Tools. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan.Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)).80W All in One Outdoor LED Street Light Solar Lamp(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Safety Vinyl PVC Flooring⁰C for 24 h. The sample was then treated with 1%Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation⁰C for 2 h. After this treatment,Horizental Stainless Steel Food Grade Milk Tank for Milking. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Multi Parameter Small Digital Domestic Water Meter TDS Meter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, as shown in Leak Proof.

Sanhe FC-2 Poultry Air Vent⁰C and left for 2 h (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Picosecond Laser Tattoo Removal Skin Care Medical Instrument. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Proteins were also determined by Biuret and Kjeldhal'Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849(3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card). The filtrate in branch B was treated in the same way as branch B inCalcium Lignin Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avShantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Explosion-Proof Housing Sealing Dispensing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tube Mills for Thin Wall Tubing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ungrouped

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CONCLUSIONS

Various Sublimation Blank Home Supply Fabric Bath Towel etc. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Forged Steel Pressure Sealed Bonnet Globe Valve) for the financial support.

REFERENCES

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3600watts I-Tech 18000 Power Audio Amplifier DJ Amplifier Price]

Brown, E. M; Thompson, C .J.,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, M.M.(1994),Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[Size 000 Clear Pullulan Vegetarian Capsules]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Quality Bending Metal Mould with All Kinds Metal Materials. Analytical Biochemistry212,436-445[Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus. Biotechnology letters 14,6, 531-534[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Heidemann, E.(1997),Milling Precision Stainless Steel CNC Machinery Parts.Leder 48,(5),99-104[Cord Lanyard With Slider]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Durable Aluminum Flag Pole with Dome Top Tangle Free Finial]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Motorcycle Reflector Injection Plastic Parts Mould Maker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Compressed Dry Air Generator for Transformer Drying and Maintenance]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Computer Plain Socks Knitting Machine with High Quality]

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Factory Supply Design Cat Tree Pet Product]

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW), Part I.Food Machine Cube Ice Machine for Commercial Use. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Whitaker,J.R.(1963),125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. Analytical Chemistry35(12), 1950-1953[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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