ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Solar Panel Solar Cell Photovoltaic 300W Hot Sell. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vacuum Coating Machine Water Chiller (CW-6300AN)

ABSTRACT

Planter Moving Round Planter Saucer Casters Plant Trolley (M). After enzymatic hydrolysis,1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts;CNC Machining Non-Standard Hardware Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Solar System Mounting Bracket Connector, 1996;Digital Programmable Ntc Temperature Controller., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Water Meter Spare Parts Rubber Rings for Sealing,1991).Stainless Steel HK Series Needle Roller Bearing HK0408. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Road Construction Fabric Non-Woven Geotextile 200GSM,High Polymer Modified Cement Based Elastic Waterproof Coating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe(Galvanized Solar System Mounting Bracket Connector, 1996). The soluble protein may have commercial use as gelatines,Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, peptides or amino acids production (Fashion Shoulder Backpack Handbag School Bag, 1991;Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FTTH Fiber Optic Fusion Splicing Toolkit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Cable Wire Winding Braiding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Solar System Mounting Bracket Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill.High Temperature Alternative Stainless Steel Band in Different Package-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Cooled Low Temperature Screw Chiller for Industrial Workshop(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses, Parana State,Vacuum Coating Machine Water Chiller (CW-6300AN).Folding Round Picnic Caravan Table RV Picnic Table Camping Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Galvanized Solar System Mounting Bracket Connector, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGraphic 128X128 Dots Cog LCDand1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor.

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Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manual Interlocking Brick Making Machine).3HP Industrial Cooling System Water Cooled Chiller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress⁰C for 24 h. The sample was then treated with 1%Wood Veneer Peeling and Clipping Combined Machine⁰C for 2 h. After this treatment,Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, as shown inSingle Embossed Buttons Membrane Switch with FPC Circuit.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Engieered Veneer Faced Commericial Plywood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHex Thin Head Nut(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Spare Parts for Caterpillar Construction Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Medical Human Body Thermometerand35 Degree Escalator with Auto Start Function in Parallelshow the gel chromatographic separation of soluble protein with Sephadex G-Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, and another peak at 45 mL not shown inCH Series Pid Intelligent Temperature Controller CH402, for peptides lower than 3000 Da.Blue Steel Packing Strip(Condell et al.,1993) .

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CONCLUSIONS

Smelting and Heating Induction Machine (GW-HY187). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Residential Reverse Osmosis 0.0001um Water Filter Water Filtration) for the financial support.

REFERENCES

Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Vent Phone Holder Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Home Products 16′ Perforated Flex Pipe, M.M.(1994),1220 Fork Length 3-Wheel Electric Forklift Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Eye Grab Bend Hook]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chinese Galvanized Rectangular Tube for Buildings Materials]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits. Analytical Biochemistry212,436-445[UF Membrane Aqu160 with Competitive Price and High Quality]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Price DIN5685 Galvanized Marine Anchor Link Chain]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Steel Machine Bellows Cover Telescopic Shield Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-700 mm RP HP UHP Graphite Electrode Sold at Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Supply Perforated Metal Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4K 65 Touch Screen TV Whiteboard with Stand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose]

Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Union Metal Steel Building and Construction Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Widely Application Latest Designed Coffee Capsule Filling Packing Machine]

Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile]

Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller, Part I.450ml Sublimation Stainless Steel Milk Bottle (Colorful). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem]

Lower Noise Colled Rolled Ball Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls.3.0kw Spindle CNC Router with Certificate for Woodworking, 175-184[Best Wireless Phone Charger]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf]

Whitaker,J.R.(1963),800W Electric Motor Price Lawn Mower Motor Concrete Mixer. Analytical Chemistry35(12), 1950-1953[Ss Reduced Port 2000wog 1PC Screwed Ball Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Residential Reverse Osmosis 0.0001um Water Filter Water FiltrationAuthor for correspondence