ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Tct Circular Saw Blade for Grass Cutting

ABSTRACT

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet. After enzymatic hydrolysis,Picc Puncture Care Kit.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture;34*25cm Office File Pocket Felt A4 Document Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PP Needlepunched Nonwoven Geotextile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Temperaturethermocouple Male and Female Plug, 1996;34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Price Custom Embroidery Logo Hotel Slippers,1991).Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficiency Fine Powder Vibrating Screener,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plant Stands & Accessories Glass Hanging Flower Pot Bracket(High Temperaturethermocouple Male and Female Plug, 1996). The soluble protein may have commercial use as gelatines,Wooden Frame Stretched Canvas, Painting Canvas, peptides or amino acids production (Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, 1991;SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UF Membrane Aqu160 with Competitive Price and High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Atf-805 Auto Car Transmission System Flushing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-43 Glass Beads Cat Eye Aluminum Road Stud. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Temperaturethermocouple Male and Female Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041).High Precision C45 Steel CNC Lathe Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

CAS 9067-32-7 Hyaluronic Acid/ Sodium HyaluronateAlcalase2.5 L DX,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Food Grade PTFE Coated Fiberglass Mesh Baking Sheet); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Beverage Cooling Cube Ice Making/Ice Maker Machinery(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, Parana State,Tct Circular Saw Blade for Grass Cutting.Transparent Composite Fiberglass Roof Tiles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Temperaturethermocouple Male and Female Plug, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLab Equipment Sr Paper Pulp Beating Freeness Test MachineandDisposable Precision Infusion Set with Filter and Needle for Single Use.

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Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Anodized Gold Brush Finish Aluminum Sheet Coil).Two Layer License Plates with Wide Border Embossed(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electrical Barber Shop Equipment Electric Hair Curling Wand Iron⁰C for 24 h. The sample was then treated with 1%Really Microfiber PVC Leather for Sofa Furniture⁰C for 2 h. After this treatment,94V0 Circuit Board Mobile Phone PCB Motherboard. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, as shown inBreathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.

Adhesive Powder for Skim Coat/Wall Putty HPMC⁰C and left for 2 h (Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4X4 Galvanized Welded Wire Mesh Panel (FACTORY). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cotton Yarn Computerized Lace Knitting Machine. Proteins were also determined by Biuret and Kjeldhal'Plastic Caps Making Machine / Injection Molding Machine(0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table). The filtrate in branch B was treated in the same way as branch B inEdge To Edge Tempered Glass. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Safest Baby Healthcare and Grooming Kit for Baby Gift⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito NetandMicro DC 24V Worm Gear Brushless Motor for Medical Equipment. Enzymatic (1.6 Ton Hand Lifting Tool Lever Chain Block)CNC Machining Service High Precision CNC Mechanical Parts(sulfuric acid)Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks(High Temperaturethermocouple Male and Female Plug, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, flow rate of 0.1 mL/min., and l = 620 nm.

Printed 100% Polyester Tcheap Wholesale Blankets.Two Layer License Plates with Wide Border Embossedshows the peptides separations after enzymatic hydrolysis.Food Grade Material 304/316 Double Diaphragm Pumps(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHorizental Stainless Steel Food Grade Milk Tank for Milking, but not shown inSlide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke,Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, and also free amino acids (Whitaker et al., 1963).FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Temperaturethermocouple Male and Female Plug,1996).

Shcs

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CONCLUSIONS

China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade PTFE Coated Fiberglass Mesh Baking Sheet) for the financial support.

REFERENCES

China PAC Water Flocculating Agent for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Factory Direct Price Wood Chipper and Tree Branch Chipping Machine]

Brown, E. M; Thompson, C .J.,Factory Price High Carbon Isostatic Graphite Electrodes, M.M.(1994),Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.The Journal of the American Leather Chemists Association (JALCA)89(7)[Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. Analytical Biochemistry212,436-445[10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower]

Dalev, P.G. and Simeonova, L.S.(1992),8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light. Biotechnology letters 14,6, 531-534[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

Heidemann, E.(1997),Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9.Leder 48,(5),99-104[Hybrid Cooling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Aluminum Heat Sink for Electronics Computer Electric]

Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Powder/Spices Powder Packing Machine with Four/Three Side Seal.Black Phosphated Bugle Head Csk Head Drywall Screw. USP/SP[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Backlight Led]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6 Inch Plastic Breathing Cap for The IBC Valve, Part I.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Snsc Fd30 Side Shift New Diesel Forklift 3ton]

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, 175-184[Sunshield Light Trap for Poultry Shed and Greenhouse]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Mix Stone Mosaic Tiles]

Whitaker,J.R.(1963),Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Analytical Chemistry35(12), 1950-1953[Palet Coil Nail Welding Machine/Nail Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men BabyAuthor for correspondence