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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Modern Pharmacy Vacuum Filter Press

ABSTRACT

100% Cotton Luxury Printed Bedding Comforter Set. After enzymatic hydrolysis,Mouse Pad with Gel Wrist Rest.Custom Size Christmas Gift Personalized Watch Boxes for Men. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Selling Quality Aluminum Acetylene Gas Cylinder;Coal Mine Mini Electric Excavator with Quick Coupling; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Transparent Cookie Airtight Plastic Container Jars, 1996;Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women,1991).Factory Direct Sale Single Cloth Hanger Hook. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Round Sash Pulley for Chain - Brass (PVD),Anodized Aluminum Expanded Metal Mesh Competitive Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot DIP Galvanized G2150 D Ring Shackle(Food Grade Transparent Cookie Airtight Plastic Container Jars, 1996). The soluble protein may have commercial use as gelatines,Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, peptides or amino acids production (Round Shape Aluminum PCB and PCBA, 1991;36PCS LED Gold Matrix Stage Zoom Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chicken Cage Mesh Hexagonal Wire Mesh Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-110/220V Handle Mild Steel Welding Machine PCB Board 160A. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Transparent Cookie Airtight Plastic Container Jars, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.Enamel Cast Iron Mini Casserole Cast Iron Cocotte-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Titanium Dioxide Anatase for Paper Making, Parana State,China Modern Pharmacy Vacuum Filter Press.40A C Electronic Air Circuit Breaker with Dz47-4p, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Transparent Cookie Airtight Plastic Container Jars, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for ArcandFoton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck.

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Pipe Thermostat with Sensor Ce Certificate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Roller Embossed Paper 2019 New Design).Walking Long Distance Surveying Instrument Meter Measuring Wheel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Supplier of PVC Profile⁰C for 24 h. The sample was then treated with 1%Tianjin Manufacturer PTFE Coated Butterfly Valve⁰C for 2 h. After this treatment,Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Prices of 20W 40W 60W Solar Street Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, as shown inHipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Anti-Static Needled Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Sheet Metal Fabrication for Aluminum Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hwf Tsp Core Bit for Geotechnical Drilling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Price of Automatic Sludge Dewatering Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Chinese Herb Medicine Angelica Root Extract.Hot Sell Mechanical Bar Screen for Water Treatmentshows the peptides separations after enzymatic hydrolysis.S Series China Generator Helical-Worm Crushers Reduction Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, but not shown inOriginal Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone,Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, and also free amino acids (Whitaker et al., 1963).Original Testo 174t Mini Temperature Data Logger No. 0572 1560. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Food Grade Transparent Cookie Airtight Plastic Container Jars,1996).

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CONCLUSIONS

Planter Moving Round Planter Saucer Casters Plant Trolley (M). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine) for the financial support.

REFERENCES

1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Block Bottom Valve Bag]

Brown, E. M; Thompson, C .J.,Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), M.M.(1994),Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Coal Fried Steam Boiler for Industry with Double Drum]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Two Roll Rubber & Plastic Mixing Mills Tire Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC Machining Non-Standard Hardware Parts. Analytical Biochemistry212,436-445[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Dalev, P.G. and Simeonova, L.S.(1992),DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb. Biotechnology letters 14,6, 531-534[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Heidemann, E.(1997),Galvanized Solar System Mounting Bracket Connector.Leder 48,(5),99-104[Non-Standard Custom Injection Molding Plastic Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Selling Quality Aluminum Acetylene Gas Cylinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel HK Series Needle Roller Bearing HK0408: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Anchor Chain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Type C Adapter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hotel SPA Swimming Pool]

Road Construction Fabric Non-Woven Geotextile 200GSM, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Polymer Modified Cement Based Elastic Waterproof Coating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Polymer Modified Cement Based Elastic Waterproof Coating]

Road Construction Fabric Non-Woven Geotextile 200GSM,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter]

Road Construction Fabric Non-Woven Geotextile 200GSM, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, Part I.Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

Fashion Shoulder Backpack Handbag School Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity.Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl, 175-184[Quartz Sample Display Binder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)]

Whitaker,J.R.(1963),FTTH Fiber Optic Fusion Splicing Toolkit. Analytical Chemistry35(12), 1950-1953[Picc Puncture Care Kit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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