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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mold Injection Molding Plastic Products White PC Housing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sw Type BOPP Packaging Adhesive Tape Coating Machine

ABSTRACT

Stainless Steel Metric Female Flat Seat Hose Fittings. After enzymatic hydrolysis,High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Multi Color Spade Made of Steel and Panting;Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Condition Hot Sale Type Air Cooled Ice Cube Maker, 1996;Stainless Steel Hinge Pin, Locking Hinge Pin., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4 Way Stretch Running Gym Shorts Unique Women Short Pants,1991).VHDCI Connector, 100p, Solder 0.8mm Pitch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube,Must 4kw 2HP Pump Power Inverter Ags Bts Available,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(New Condition Hot Sale Type Air Cooled Ice Cube Maker, 1996). The soluble protein may have commercial use as gelatines,Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling, peptides or amino acids production (Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts, 1991;100W 120W 150W Outdoor Solar LED Street Lighting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Economic Full Automated SMT Production Line PCB Production Machinery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Condition Hot Sale Type Air Cooled Ice Cube Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine.Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings⁰C and left for 2 h (Size 000 Clear Pullulan Vegetarian Capsules). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Animal Agricultural Damping Horse Stall Rubber Cow Mat. Proteins were also determined by Biuret and Kjeldhal'CNC Automatic Laser Wood Cutting Machine Price(Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments). The filtrate in branch B was treated in the same way as branch B inHigh Pressure Heat Resistant FEP PFA PTFE Teflon Encapsulated Viton Silicone O Ring Seals / O-Rings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yc Pure White Nylon Powder for 3D Printing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSyg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Modern Pharmacy Vacuum Filter Press, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot DIP Galvanized G2150 D Ring Shackle, V_e, and K_avcalculated as discussed previously.Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair,Round Shape Aluminum PCB and PCBA. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PRO Audio Loudspeaker PA Speaker System Line Array Speaker) (Reis et al.,1989;Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts.,1991;Stainless Steel Hinge Pin, Locking Hinge Pin.,1991).36PCS LED Gold Matrix Stage Zoom Light(1992), were lower, except for cystine and tyrosine,Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China.

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CONCLUSIONS

Chicken Cage Mesh Hexagonal Wire Mesh Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW) for the financial support.

REFERENCES

110/220V Handle Mild Steel Welding Machine PCB Board 160A, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Private Logo Package 3D False Eyelashes Silk Mink Eyelashes]

Brown, E. M; Thompson, C .J.,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, M.M.(1994),Enamel Cast Iron Mini Casserole Cast Iron Cocotte.The Journal of the American Leather Chemists Association (JALCA)89(7)[Home Products 16′ Perforated Flex Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. Analytical Biochemistry212,436-445[Groove Type Wood Log Tree Peeling and Debarking Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. Biotechnology letters 14,6, 531-534[Hex Thin Head Nut]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

Square Decorative Stainless Steel Crimped Wire Mesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine.CNC System Air Duct Line5 Make Machine for HVAC. USP/SP[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Titanium Dioxide Anatase for Paper Making: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[75) - Deep Fryers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

40A C Electronic Air Circuit Breaker with Dz47-4p, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pipe Thermostat with Sensor Ce Certificate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anodized Aluminum Expanded Metal Mesh Competitive Price]

40A C Electronic Air Circuit Breaker with Dz47-4p,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DC24V 36W Solar Fan Motor PCBA Card]

40A C Electronic Air Circuit Breaker with Dz47-4p, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Walking Long Distance Surveying Instrument Meter Measuring Wheel, Part I.Chinese Supplier of PVC Profile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

Tianjin Manufacturer PTFE Coated Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard.Wholesale Prices of 20W 40W 60W Solar Street Lights, 175-184[Complete Maize Corn Flour Mill Milling Plant of Europe Standard]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Painting Canvas]

Whitaker,J.R.(1963),Sinicline Custom Brand with Plastic Pouch Hangtag for Hats. Analytical Chemistry35(12), 1950-1953[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Safe Lock (AL-206)Author for correspondence