ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Washami Teeth Care for Whitening Cool Mint Toothpaste. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope

ABSTRACT

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. After enzymatic hydrolysis,SHXJ1500 CNC Diamond Wire Stone Cutting Machine.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2019 Foam Concrete Machine/Foamed Concrete Block Making Machine;Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yyh Series One Dimension Tumbling Mixer Mixing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ODU 14 Pin Industrial Circular Connector, 1996;Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Classical New Coming Plastic Female Sunglasses,1991).OEM Double Sided Rigid PCB Circuit Board Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Robot of World Robotics Competition,Professional Rope/Cable/Wire Braiding Machine with Factory Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum PCB Circuit Board SMD PCBA LED PCB(ODU 14 Pin Industrial Circular Connector, 1996). The soluble protein may have commercial use as gelatines,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), peptides or amino acids production (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 1991;China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Concrete Cure Spray Concrete Curing Agent., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Chewing Dog Food Snack Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White Ceramic Shot Glass for Sublimation Printing - 1.5oz. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ODU 14 Pin Industrial Circular Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Promotional Gifts Custom Printed Silicone Wristband/Bracelet.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Farrowing Crate Pig Floor Cast Iron Slat FloorAlcalase2.5 L DX,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality Aluminum Heat Sink for Electronics Computer Electric); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Surprise! Range Hood Free! ! ! Juice Cart, Parana State,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope.Steam Eye Mask Heating One Eye Mask for Sleeping, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ODU 14 Pin Industrial Circular Connector, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStrong Motor Permanent Neodymium Rare Earth Bar Magnet N52andCustomized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label.

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Automatic French Fries Frying Machine with Low Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet).Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software⁰C for 24 h. The sample was then treated with 1%Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security⁰C for 2 h. After this treatment,Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, as shown in60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light.

T90 High Power Latching Relay Mini Power Relay⁰C and left for 2 h (Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. Proteins were also determined by Biuret and Kjeldhal'Polyglycolic Acid Absorable Suture(Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature). The filtrate in branch B was treated in the same way as branch B inPlastic and Metal/ Aluminium Parts Machining/ CNC Machining Parts OEM. The final soluble protein shown were used for peptides separations using gel permeation chromatography,No. 1062 20PCS Tools.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Mould Shoe Mould Making CNC Milling Router Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Refrigeration Digital Temperature Controller Stc-100A, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PCB18h 8p8c Modular Plug with Shield Head RJ45 ConnectorandWomen Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies. Enzymatic (Black Color Hexagon Bolt with High Quality)China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes(sulfuric acid)Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price(ODU 14 Pin Industrial Circular Connector, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, flow rate of 0.1 mL/min., and l = 620 nm.

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Setshows the peptides separations after enzymatic hydrolysis.Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Black Galvanized Head Stainless Steel Screw, but not shown inTop Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006,Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, and also free amino acids (Whitaker et al., 1963).Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ODU 14 Pin Industrial Circular Connector,1996).

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Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, V_e, and K_avcalculated as discussed previously.Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads,Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro) (Reis et al.,1989;Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck.,1991;Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board.,1991).Prefab Expandable Luxury Living Container House Prefabricated House(1992), were lower, except for cystine and tyrosine,Movable Partition Walls for Room Division, Hotel and Shopping Mall.

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CONCLUSIONS

Carboxy Terminal Collagen Crosslinks Elisa Kits. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Aluminum Heat Sink for Electronics Computer Electric) for the financial support.

REFERENCES

Various Colors Nylon and Stainless Steel Kitchen Tools, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Part Casting Suppliers for Sale]

Brown, E. M; Thompson, C .J.,355nm UV Laser Marking Equipment for Crystal Materials, M.M.(1994),Metal Separating Gold Brass Zippers for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Striped Compression Soccer Socks for Adults]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Analytical Biochemistry212,436-445[Low Price Sweep Frequency Response Analyzer for Power Transformer Test]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Refrigeration Digital Thermometer. Biotechnology letters 14,6, 531-534[China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA]

Heidemann, E.(1997),BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).Leder 48,(5),99-104[5tons Wheel Loader]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

1220*2440mm Concrete Formwork black film faced plywood, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making.79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. USP/SP[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Two Roll Rubber & Plastic Mixing Mills Tire Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale 25kw Grid Tied Home Solar Panel System Kit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini Car Portable Fridge 10L Mini Fridge, Part I.Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

Mini Small Garden Excavator Digging Work, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver.Offshore Drilling Platform Air Winch for Marine Oilfields, 175-184[Black Painted Glass]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non Standard U Channel Steel]

Whitaker,J.R.(1963),Mass Stock Yl10.2 Carbide Rods for Milling 45HRC. Analytical Chemistry35(12), 1950-1953[High Speed Copper Wire Braiding Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)Author for correspondence