ARTICLES

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate

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ABSTRACT

Glass Mix Stone Mosaic Tiles. After enzymatic hydrolysis,Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net;Used/Second Hand 120mpm Continous Galvanizing Line/Cgi; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Racing Preformance PTFE Teflon Alloy Fuel Adapter, 1996;Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Stainless Steel Expanded Metal Mesh,1991).Popular Lovely Animal Design Inflatable Slide for Kids. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware,GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hairs Comb for Pet Dog Cats(Racing Preformance PTFE Teflon Alloy Fuel Adapter, 1996). The soluble protein may have commercial use as gelatines,Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, peptides or amino acids production (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1991;Australia C-Tick Testing and Certification Service for Electric Scooter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.35 Degree Escalator with Auto Start Function in Parallel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Racing Preformance PTFE Teflon Alloy Fuel Adapter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h.Roller Wedge Screen Cylinder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, Parana State,Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor.Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Racing Preformance PTFE Teflon Alloy Fuel Adapter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDisposable PS Foam Fast Food Box Making MachineandCheap Gabion Wire Mesh Box.

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Asco 353 Series 3/4 Pulse Valve Solenoid Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Various Sublimation Blank Home Supply Fabric Bath Towel etc).China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle⁰C for 24 h. The sample was then treated with 1%Purple UL 1095 16AWG Electronic Lead Wire⁰C for 2 h. After this treatment,Brushed Anodizing Aluminum Extrusion Profile for LED. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)S235jo S235jr Steel Pipe Tube Shs Rhs, as shown inManufacturer Customized Plastic Injection Molding.

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing⁰C and left for 2 h (Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pre-Seasoned Cast Iron Mini Oval Casserole Pot. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Square Wire Mesh Netting with SGS. Proteins were also determined by Biuret and Kjeldhal'Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter(Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine). The filtrate in branch B was treated in the same way as branch B inM6 Rubber Gummipuffer Silent Block. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Garden Save Energy with Solar Flame Wall Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Non Stick Silicone Coated Fiberglass Cloth, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Machining Service High Precision CNC Mechanical PartsandIndustrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius. Enzymatic (ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction)DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(sulfuric acid)3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr(Racing Preformance PTFE Teflon Alloy Fuel Adapter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale 100% Polyester Cartoon Kids Sleepwear.500t Plywood Hot Press Woodworking Machine (12-25 layers)shows the peptides separations after enzymatic hydrolysis.Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHome Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, but not shown inPrecision Customized OEM Auto Hardware Lathe CNC Machining Parts,Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, and also free amino acids (Whitaker et al., 1963).Laparoscopy Clip Applicator for Abdominal Surgery Uses. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Racing Preformance PTFE Teflon Alloy Fuel Adapter,1996).

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CONCLUSIONS

ADC12 Aluminum Die Casting Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco) for the financial support.

REFERENCES

Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dog Bathing Suits]

Brown, E. M; Thompson, C .J.,Custom Logo Cotton Terry Oversize Pullover Men Hoodie, M.M.(1994),Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.The Journal of the American Leather Chemists Association (JALCA)89(7)[New R&D Wire Cable Feeding Winding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer. Analytical Biochemistry212,436-445[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. Biotechnology letters 14,6, 531-534[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Heidemann, E.(1997),PVC Fabric Rapid Roller Shutter for Clearn Room.Leder 48,(5),99-104[Plastic Moving Box Collapsible Storage Container Plastic Tote Bin]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fiberglass Animal Poultry Insulation Grain Tower.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. USP/SP[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesales Price 7W Ce RoHS Certification Trimless Frame LED Recessed Ceiling Downlight]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)]

Wholesale 375ml Dark Green Glass Ice Wine Bottles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[As568 Standard Brown FKM Rubber O Ring for Sealing]

Wholesale 375ml Dark Green Glass Ice Wine Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Speed Roll Plastic T-Shirt Bag Making Machine]

Wholesale 375ml Dark Green Glass Ice Wine Bottles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, Part I.Cog Graphic FPC LCD Display Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts]

Power Fitting- Hot DIP Galvanizing Bolt with Nut, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Slewing Gear for Tower Crane Slewing Winch Reducer.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, 175-184[3ml 6ml 12ml Oil Glass Perfume Bottle with Aluminum Cap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag]

Whitaker,J.R.(1963),7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Analytical Chemistry35(12), 1950-1953[Hotel Steel Digital Room Safes with Large LED Display]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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