ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Di DIN3352 Double Orifice Automatic Air Release Valve Dn65. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Magic Hair Twist   Curl Sponge Brush

ABSTRACT

Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). After enzymatic hydrolysis,Geyi Disposable Laparoscopic Visible Optical Trocar.Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (175mm Diamond Cutting Blade for Granite&Tiles, 1996;Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings,1991).Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Uprigth Touch-Screen Red Wine Chiller,Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder(175mm Diamond Cutting Blade for Granite&Tiles, 1996). The soluble protein may have commercial use as gelatines,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, peptides or amino acids production (Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, 1991;Groove Type Wood Log Tree Peeling and Debarking Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality CE Digital Display Adjustable Power Supply., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Parcel Tape Adhesive OPP Packing Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (175mm Diamond Cutting Blade for Granite&Tiles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Non-Expensive Cracking Agent.Fiber Optic Sc APC Fast Connector for FTTH Drop Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, Parana State,Magic Hair Twist   Curl Sponge Brush.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (175mm Diamond Cutting Blade for Granite&Tiles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFolding Play Mat Crawl Play MatandDbry-320 Christmas Stickers Labels Printing Machine.

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Best Price PVC Coated Fiberglass Mesh Insect Window Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fiber Optic Sc APC Fast Connector for FTTH Drop Cable).Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor⁰C for 24 h. The sample was then treated with 1%Factory OEM CNC Machined Gear Motor Shaft⁰C for 2 h. After this treatment,High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pink French Loop Terry Knit Fabric for Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, as shown inSelf Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key.

Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts⁰C and left for 2 h (Aluminum Foil PE Bottle Cap Induction Seal Liner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. Proteins were also determined by Biuret and Kjeldhal'Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)(Racing Preformance PTFE Teflon Alloy Fuel Adapter). The filtrate in branch B was treated in the same way as branch B inNew Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCharateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3kw 24V 310mm Vertical Agv Drive Wheel Assembly⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sofa Materials PU Leather with Flock Backing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Three Phase 800mm Plate Fan, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Gear Rolling Machine Zh28/25 Spline Hobbing Machine.Robot of World Robotics Competitionshows the peptides separations after enzymatic hydrolysis.OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2mm Mirror Glass/Coated Glass for LED, but not shown inFood Machine Cube Ice Machine for Commercial Use,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, and also free amino acids (Whitaker et al., 1963).Partial Thread Steel/Iron Hex Head Self Tapping Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (175mm Diamond Cutting Blade for Granite&Tiles,1996).

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CONCLUSIONS

on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen) for the financial support.

REFERENCES

Stand Hot and Cold Normal Water Dispenser with Refrigerator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Cooling Spare Parts Compressor Magnetic Clutch]

Brown, E. M; Thompson, C .J.,High Quality Construction Welding Rods Electrode E6013 E7018 E7016, M.M.(1994),High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.The Journal of the American Leather Chemists Association (JALCA)89(7)[55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular. Analytical Biochemistry212,436-445[OEM Refrigeration Digital Thermometer]

Dalev, P.G. and Simeonova, L.S.(1992),Lydite Fence Reel Reel with Extension Arms for Farm Fencing. Biotechnology letters 14,6, 531-534[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Heidemann, E.(1997),1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio.Leder 48,(5),99-104[Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Strength New 0.18mm Molybdenum Wire]

Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized 3D Medal for Religious Medal Gift.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. USP/SP[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Precision Infusion Set with Filter and Needle for Single Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dbry-320 Christmas Stickers Labels Printing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ycom USB Charger and Data Cable with Aluminum for Type C]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acrylic Backing DIY LED Backlit Channel Letter Sign]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anodized Gold Brush Finish Aluminum Sheet Coil]

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, Part I.Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table]

Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope.High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, 175-184[Luxury Handmade Natural Fragrance 100% Soy Wax Candle in Black Glass Jar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net]

Whitaker,J.R.(1963),High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. Analytical Chemistry35(12), 1950-1953[Two Layer License Plates with Wide Border Embossed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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