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ABSTRACT

Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers. After enzymatic hydrolysis,Stainless Steel Filter Strainer.Granite Cutting Diamond Saw Blades Cutting Blade. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Steel Forged Automobile Spare Parts with Customized Drawing;Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, 1996;T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine,1991).Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mechanical Seal 101 Type for Pump and Auto,Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass(Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, 1996). The soluble protein may have commercial use as gelatines,China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, peptides or amino acids production (2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, 1991;Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Pressure PU Filter Gasket Sandal Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook.Flour Making Machine Wheat Maize Flour Mill Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Residence Use 220V 50Hz 12kw Portable silent Diesel Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGarden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Roller Shutter Window Torsion Springs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment) for the financial support.

REFERENCES

Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dongfeng 5cbm Mini Water Tank Truck]

Brown, E. M; Thompson, C .J.,Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, M.M.(1994),Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Sheet Metal Fabrication for Aluminum Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Groove Type Wood Log Tree Peeling and Debarking Machine. Analytical Biochemistry212,436-445[Used/Second Hand 120mpm Continous Galvanizing Line/Cgi]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality CE Digital Display Adjustable Power Supply. Biotechnology letters 14,6, 531-534[Cabinet Shelf Support Pin Furniture Hardware Fittings]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fiber Optic Sc APC Fast Connector for FTTH Drop Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Square Decorative Stainless Steel Crimped Wire Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Smart Boards For Business]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chemicals Flocculant Cationic Polymer Price]

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Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ JIS G 3461 ASTM A179 Seamless Boiler Pipe]

Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), Part I.Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening]

Whitaker,J.R.(1963),Best Price PVC Coated Fiberglass Mesh Insect Window Screen. Analytical Chemistry35(12), 1950-1953[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q PowerampAuthor for correspondence