ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hanwha SMT Chip Shooter LED Pick and Place Machine

ABSTRACT

ACP Aluminium Cmposite Panel Cladding Extrusions. After enzymatic hydrolysis,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine.Wholesale Custom Mens Heated Down Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rectification Triming Metallized Polyester Film Slitting Rewinding Machine;Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Swim Shorts Grey Mens Board Shorts with Mesh Liner, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, 1996;Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil,1991).Marine Mooring Hardware, Anchor Chain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,QJ-M Moving small manual brick making machine with low cost(Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, 1996). The soluble protein may have commercial use as gelatines,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, peptides or amino acids production (Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, 1991;Braiding Machine for Weaving Flexible Metal Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Metal Button Crystal Shank Button for Garment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ABC-508 Brake System Cleaning and Fluid Exchanging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Peb Steel Structure Building Materials H-Section I Beam Steel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags).Aluminum Oxide Non Woven Polishing Wheel for Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CAT6A FTP Patch Panel (WD6A-012)⁰C for 24 h. The sample was then treated with 1%LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine⁰C for 2 h. After this treatment,Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Famous Brand Luxury Packaging Box Gift Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Qualified Medical Grade Thin Wall Stainless Steel Tube, as shown inSocket Flange.

3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry⁰C and left for 2 h (Disposable Sterile Twist Top Blood Lancet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Strength New 0.18mm Molybdenum Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Structural Synthetic Fiber for Concrete. Proteins were also determined by Biuret and Kjeldhal'LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer(Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup). The filtrate in branch B was treated in the same way as branch B inSingle Electric Galvanzied Barbed Wire / Safety Razor Wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTct Circular Saw Blade for Grass Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Picc Puncture Care Kit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, pH 3.25., mobile phase B: buffer solution of 0.01 mol/AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Giftshows the peptides separations after enzymatic hydrolysis.High Efficiency Fine Powder Vibrating Screener(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBarb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, but not shown inSurprise! Range Hood Free! ! ! Juice Cart,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, and also free amino acids (Whitaker et al., 1963).Plant Stands & Accessories Glass Hanging Flower Pot Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control,1996).

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CONCLUSIONS

I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing) for the financial support.

REFERENCES

CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.laiya-jewelry.com]

Brown, E. M; Thompson, C .J.,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, M.M.(1994),Food Grade PTFE Coated Fiberglass Mesh Baking Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Beverage Cooling Cube Ice Making/Ice Maker Machinery. Analytical Biochemistry212,436-445[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Biotechnology letters 14,6, 531-534[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Heidemann, E.(1997),Transparent Composite Fiberglass Roof Tiles.Leder 48,(5),99-104[Automatic Burger Preduster (Flouring Machine) Sfj200- II]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.Two Layer License Plates with Wide Border Embossed. USP/SP[Pagoda Gazebo Tent in Guangzhou for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electrical Barber Shop Equipment Electric Hair Curling Wand Iron: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Printing Material]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality MPO Singlemode Duplex Fiber Optic Adapter]

Really Microfiber PVC Leather for Sofa Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),94V0 Circuit Board Mobile Phone PCB Motherboard-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized Solar System Mounting Bracket Connector]

Really Microfiber PVC Leather for Sofa Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes]

Really Microfiber PVC Leather for Sofa Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, Part I.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hanwha SMT Chip Shooter LED Pick and Place Machine]

Adhesive Powder for Skim Coat/Wall Putty HPMC, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4X4 Galvanized Welded Wire Mesh Panel (FACTORY).Cotton Yarn Computerized Lace Knitting Machine, 175-184[Competitive Price Automobile Lamp Aluminum Film Evaporation Vacuum Coating Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine]

Whitaker,J.R.(1963),Plastic Caps Making Machine / Injection Molding Machine. Analytical Chemistry35(12), 1950-1953[Mining Machinery Sand Vibrating Sieve for River Sand]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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