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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Glass Cutting Table/CNC Glass Cutting Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,12 Bottles Portable Home Ues Thermoelectric Wine Chiller

ABSTRACT

Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter. After enzymatic hydrolysis,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321).Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1996;Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron,1991).Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt,Hanwha SMT Chip Shooter LED Pick and Place Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ACP Aluminium Cmposite Panel Cladding Extrusions(Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1996). The soluble protein may have commercial use as gelatines,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, peptides or amino acids production (Wholesale Custom Mens Heated Down Jacket, 1991;Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Swim Shorts Grey Mens Board Shorts with Mesh Liner.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization).Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Marine Mooring Hardware, Anchor Chain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for GirlsAlcalase2.5 L DX,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-QJ-M Moving small manual brick making machine with low cost); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, Parana State,12 Bottles Portable Home Ues Thermoelectric Wine Chiller.Braiding Machine for Weaving Flexible Metal Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBlack Phosphated Bugle Head Csk Head Drywall ScrewandVinyl Glove in Work & Labor Glove.

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Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop).Fashion Metal Button Crystal Shank Button for Garment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)⁰C for 24 h. The sample was then treated with 1%10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light⁰C for 2 h. After this treatment,Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ABC-508 Brake System Cleaning and Fluid Exchanging Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polypropylene Woven Bag for Sludge Dewatering, as shown inMovable Partition Walls for Room Division.

China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale⁰C and left for 2 h (Home and Hospital Electric Patient Lift). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Industrial Grade Anhydrous Sodium Sulfate. Proteins were also determined by Biuret and Kjeldhal'Professional Factory Supply Design Cat Tree Pet Product(Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle). The filtrate in branch B was treated in the same way as branch B inRainbow Neon Iridescent Reflective Fabric Printed for Sun-Proof Clothing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Peb Steel Structure Building Materials H-Section I Beam Steel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Oxide Non Woven Polishing Wheel for Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Famous Brand Luxury Packaging Box Gift Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling RigandHigh Qualified Medical Grade Thin Wall Stainless Steel Tube. Enzymatic (3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry)High Strength New 0.18mm Molybdenum Wire(sulfuric acid)Structural Synthetic Fiber for Concrete(Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, flow rate of 0.1 mL/min., and l = 620 nm.

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34*25cm Office File Pocket Felt A4 Document Bag, V_e, and K_avcalculated as discussed previously.PP Needlepunched Nonwoven Geotextile,High Temperaturethermocouple Male and Female Plug. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (The Most Competitive Sodium Gluconate for Water Reducer) (Reis et al.,1989;Wholesale Custom Mens Heated Down Jacket.,1991;Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve.,1991).34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts(1992), were lower, except for cystine and tyrosine,Best Price Custom Embroidery Logo Hotel Slippers.

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CONCLUSIONS

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (QJ-M Moving small manual brick making machine with low cost) for the financial support.

REFERENCES

High Efficiency Fine Powder Vibrating Screener, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kids Soccer Gate Net]

Brown, E. M; Thompson, C .J.,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, M.M.(1994),Plant Stands & Accessories Glass Hanging Flower Pot Bracket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Machine for Making]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wooden Frame Stretched Canvas, Painting Canvas. Analytical Biochemistry212,436-445[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine. Biotechnology letters 14,6, 531-534[Steam Eye Mask Heating One Eye Mask for Sleeping]

Heidemann, E.(1997),SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.Leder 48,(5),99-104[4.8mm/6.3mm/Brass Round Pin Plug Pin]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

China Supplier Granular Coal Based Activated Carbon Filter Meida, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UF Membrane Aqu160 with Competitive Price and High Quality.Atf-805 Auto Car Transmission System Flushing Machine. USP/SP[Banded Type Tee Used on Oil]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-43 Glass Beads Cat Eye Aluminum Road Stud: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pet Anti-Static Needled Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oxygen Mask with Reservoir Bag/ with Nebulizer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Black Small Fridge for Showcase]

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Precision C45 Steel CNC Lathe Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, Part I.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Food Grade PTFE Coated Fiberglass Mesh Baking Sheet.Beverage Cooling Cube Ice Making/Ice Maker Machinery, 175-184[Eva Foam Stone Sample Display Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Beverage Cooling Cube Ice Making/Ice Maker Machinery]

Whitaker,J.R.(1963),Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Analytical Chemistry35(12), 1950-1953[30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Magic Hair Twist   Curl Sponge BrushAuthor for correspondence