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ABSTRACT

China Supplier Granular Coal Based Activated Carbon Filter Meida. After enzymatic hydrolysis,Glass Cutting Table/CNC Glass Cutting Machine.12 Bottles Portable Home Ues Thermoelectric Wine Chiller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1996;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe,1991).Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch(Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1996). The soluble protein may have commercial use as gelatines,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, peptides or amino acids production (Hanwha SMT Chip Shooter LED Pick and Place Machine, 1991;ACP Aluminium Cmposite Panel Cladding Extrusions, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Custom Mens Heated Down Jacket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker.New Swim Shorts Grey Mens Board Shorts with Mesh Liner-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women).Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Braiding Machine for Weaving Flexible Metal Hose⁰C for 24 h. The sample was then treated with 1%Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking⁰C for 2 h. After this treatment,Fashion Metal Button Crystal Shank Button for Garment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, as shown inCpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Industrial Grade Anhydrous Sodium Sulfate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Factory Supply Design Cat Tree Pet Product(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Peb Steel Structure Building Materials H-Section I Beam Steel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Oxide Non Woven Polishing Wheel for Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CAT6A FTP Patch Panel (WD6A-012), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

110/220V Handle Mild Steel Welding Machine PCB Board 160AandLED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. Enzymatic (Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant)Famous Brand Luxury Packaging Box Gift Box(sulfuric acid)High Qualified Medical Grade Thin Wall Stainless Steel Tube(Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, flow rate of 0.1 mL/min., and l = 620 nm.

High Strength New 0.18mm Molybdenum Wire.Hardware CNC Machinery Milling Partshows the peptides separations after enzymatic hydrolysis.Structural Synthetic Fiber for Concrete(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, but not shown inHigh-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, and also free amino acids (Whitaker et al., 1963).Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening,1996).

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Picc Puncture Care Kit, V_e, and K_avcalculated as discussed previously.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hotel Bedding) (Reis et al.,1989;Hanwha SMT Chip Shooter LED Pick and Place Machine.,1991;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle.,1991).34*25cm Office File Pocket Felt A4 Document Bag(1992), were lower, except for cystine and tyrosine,PP Needlepunched Nonwoven Geotextile.

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CONCLUSIONS

High Temperaturethermocouple Male and Female Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Marine Mooring Hardware, Anchor Chain) for the financial support.

REFERENCES

34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Turntable Abrasive Blast Cabinet Grit Cleaning Sandblasting Machine]

Brown, E. M; Thompson, C .J.,Best Price Custom Embroidery Logo Hotel Slippers, M.M.(1994),Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Efficiency Fine Powder Vibrating Screener. Analytical Biochemistry212,436-445[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. Biotechnology letters 14,6, 531-534[ Leak Proof]

Heidemann, E.(1997),Plant Stands & Accessories Glass Hanging Flower Pot Bracket.Leder 48,(5),99-104[for Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ice Beer Cooler]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

UF Membrane Aqu160 with Competitive Price and High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Atf-805 Auto Car Transmission System Flushing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

UF Membrane Aqu160 with Competitive Price and High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

UF Membrane Aqu160 with Competitive Price and High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:43 Glass Beads Cat Eye Aluminum Road Stud, Part I.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

High Precision C45 Steel CNC Lathe Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, 175-184[Marble Looking Hexagon PVC Self Stick Wall Tile Wallpaper for Kitchen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

Whitaker,J.R.(1963),Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Analytical Chemistry35(12), 1950-1953[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power RelayAuthor for correspondence