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ABSTRACT

Hotel Bedding. After enzymatic hydrolysis,Supplier V Wedge Wire Johnson Water Well Screen.Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1996;Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line,1991).Factory Outlet Automatic Grain Tower Dryer Drying Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mesh Bag Packing 20kg Pure White Garlic,Supreme 524 Mv Sea Kayak,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Trunk Organizer Foldable Water Proof(60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1996). The soluble protein may have commercial use as gelatines,100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), peptides or amino acids production (Diesel Generator Parts AVR Ki-Davr-95s for Kipor, 1991;Gld Brand Padlock Brass Popular in 5 Continents  , 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;No Lead Bronze Flange Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Snsc Fd30 Side Shift New Diesel Forklift 3ton. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Laptop Bag College Men Backpack Leather Back Pack Bags(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, Parana State,High Quality MPO Singlemode Duplex Fiber Optic Adapter.Automatic Food Metal Detector Machine for Production Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNa2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing AgentandHumidity Refrigeration Digital Controller Jsd-100+.

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Multi-Functional Backpack Manual 52cc Brush Cutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nylon Weatherproof PA6 Strain Relief Cable Gland).Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine⁰C for 24 h. The sample was then treated with 1%Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm⁰C for 2 h. After this treatment,White Cotton Satin Hotel Duvet Cover (DPFB8055). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Cleaning Brush ESD Anti Static Brush ESD Brush Black's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PAC /Poly Aluminium Chloride for Water Treatment, as shown inAluminum Bright Polished Mirror Sheet.

Aluminum Sheet Shearing Machine⁰C and left for 2 h (Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electrical PV DC Solar Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tomato Bubble and High Pressure Washing Machine. Proteins were also determined by Biuret and Kjeldhal'Upholstery Ribbon Christmas Tape(Electrical Barber Shop Equipment Electric Hair Curling Wand Iron). The filtrate in branch B was treated in the same way as branch B in2ft Batten Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2+1 Flavors Soft Ice Cream Machine with Pre-Cooling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCosta Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Sale Low Price Household Air freshener Spray⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supplied High Strength Marine Anchor Chain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Humidity Refrigeration Digital Controller Jsd-100+, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

HDPE Polyethylene PE Pipe for Water SupplyandElegant Europe Dessign Pillow Top Mattress. Enzymatic (8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8))Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts(sulfuric acid)Modern 4 Person Workstation with Computer Desk Modular Office Furniture(60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill, flow rate of 0.1 mL/min., and l = 620 nm.

Women Fashion Winter V Neck Long Sleeve Clothes.Chinese Supplier of PVC Profileshows the peptides separations after enzymatic hydrolysis.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNon Rechargeable, but not shown inHot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001),3D CNC Router for Wood Cutting Machine, and also free amino acids (Whitaker et al., 1963).Stainless Steel Screens / Wedge Wire Filter Element. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (60ml 100ml Customized Glass Jar for Spice, Candle, Candy,1996).

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CONCLUSIONS

High Quality Low Price Top Selling Fashion Summer Boy Sandals. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery) for the financial support.

REFERENCES

Easy Operational Cup Cake Filling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Design Jacquard Men Compression Sport Basketball Socks]

Brown, E. M; Thompson, C .J.,Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger, M.M.(1994),Folding Play Mat Crawl Play Mat.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wooden Grain Perforated Panel for Metal Mesh Curtain Wall. Analytical Biochemistry212,436-445[High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Laundry Ozone Generator for Jeans]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Purple UL 1095 16AWG Electronic Lead Wire]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Powerful Cleaning Colorful Scouring Pad for Kitchen Use]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Perforated Coils]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Popular Laser Cutting Engraving Machine for Nonmetals]

Low Carbon Heavy Galvanized Steel Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Long Distance Measurement Power Laser Digital Meter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dry Air Generator GF Series]

Low Carbon Heavy Galvanized Steel Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent]

Low Carbon Heavy Galvanized Steel Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer, Part I.Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing]

Whitaker,J.R.(1963),High Quality CO2 Laser Marking Machine with Ce. Analytical Chemistry35(12), 1950-1953[PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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