ARTICLES

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ABSTRACT

Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber. After enzymatic hydrolysis,Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand.Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fully Automatic Dust Face Mask Making Machine with Ultrasonic, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, 1996;Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle,1991).Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Bb Trumpet (ATR6506)(3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, 1996). The soluble protein may have commercial use as gelatines,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, peptides or amino acids production (3.5ton Chinese Engine Gasoline Forklift (FG35T), 1991;Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof Covering Material Interior Wall Composite Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tall/Large Cube Glass Terrarium Planter Gift Card Holder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Venlo Type Flower Multi Span Commercial Glass Greenhouses Used.XPS Extruded Polystyrene Foam Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Electric Heavy Duty Rail Guided Vehicle for Transportation, Parana State,External Retaining Ring for Shaft, DIN471, D1400.Sower Vacuum Emulsifying Machine for Cosmetic, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting MachineandStainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price.

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ASTM A240 309s Stainless Steel Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine).Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Car Mattress Back Inflatable Air Bed Car⁰C for 24 h. The sample was then treated with 1%24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum⁰C for 2 h. After this treatment,Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single Embossed Buttons Membrane Switch with FPC Circuit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, as shown inHigh-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chemicals Flocculant Cationic Polymer Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYcom USB Charger and Data Cable with Aluminum for Type C(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Light Weight Polyester Eye Mask for Travel Airplane Hotel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Silver Anodized Aluminium I Beam Level Measuring Tool. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Deep Hole Cylinder Boring Machine) for the financial support.

REFERENCES

Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20meter Air Hose Reel]

Brown, E. M; Thompson, C .J.,48V25A Lead Acid Battery Maintenance Pulse Charger, M.M.(1994),15mm Best Price Commercial Grade Birch Plywood for Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[V-32 New Special UPS Solar Battery Cabinet Box Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Great Wallet Genuine Leather Purse with Coin Pocket. Analytical Biochemistry212,436-445[Seikofire S-66 Palm OTDR Sm/mm Fiber Testing]

Dalev, P.G. and Simeonova, L.S.(1992),Good Price 20kVA Single Phase Silent Diesel Generator. Biotechnology letters 14,6, 531-534[Single Stage High Speed Fruit and Vegetable Pulping Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Coal Mine Mini Electric Excavator with Quick Coupling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Eaton 6k Orbit Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, Part I.Stainless Steel Clarifier Tank for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Price of a Retail Supermarket Push Cart for Sale]

Carrier Roller for Sany Excavator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes.Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 175-184[4m Outdoor Luxury Inflatable Camping Bubble Tent Clear Acrylic Air Dome Tents]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Whitaker,J.R.(1963),Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts. Analytical Chemistry35(12), 1950-1953[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wet Type Wire Drawing Machine for Carbon Steel and Stainless SteelAuthor for correspondence