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ABSTRACT

Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. After enzymatic hydrolysis,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.Hanwha SMT Chip Shooter LED Pick and Place Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ACP Aluminium Cmposite Panel Cladding Extrusions;New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Custom Mens Heated Down Jacket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, 1996;Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Swim Shorts Grey Mens Board Shorts with Mesh Liner,1991).Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization),Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Marine Mooring Hardware, Anchor Chain(Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, 1996). The soluble protein may have commercial use as gelatines,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, peptides or amino acids production (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1991;QJ-M Moving small manual brick making machine with low cost, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Braiding Machine for Weaving Flexible Metal Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.Fashion Metal Button Crystal Shank Button for Garment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Industrial Grade Anhydrous Sodium Sulfate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG).Professional Factory Supply Design Cat Tree Pet Product(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)⁰C for 24 h. The sample was then treated with 1%Peb Steel Structure Building Materials H-Section I Beam Steel⁰C for 2 h. After this treatment,Aluminum Oxide Non Woven Polishing Wheel for Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CAT6A FTP Patch Panel (WD6A-012)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, as shown in 800 Wog.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Structural Synthetic Fiber for Concrete. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tct Circular Saw Blade for Grass Cutting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

43 Glass Beads Cat Eye Aluminum Road Stud. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ABC-508 Brake System Cleaning and Fluid Exchanging Machine) for the financial support.

REFERENCES

Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Apollo 12 Full Spectrum LED Grow Lamp with Ce FCC RoHS]

Brown, E. M; Thompson, C .J.,High Precision C45 Steel CNC Lathe Parts, M.M.(1994),I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. Analytical Biochemistry212,436-445[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

Dalev, P.G. and Simeonova, L.S.(1992),Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Biotechnology letters 14,6, 531-534[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Heidemann, E.(1997),Food Grade PTFE Coated Fiberglass Mesh Baking Sheet.Leder 48,(5),99-104[Swim Fins Silicone Unisex]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Beverage Cooling Cube Ice Making/Ice Maker Machinery.Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. USP/SP[Light Weight Polyester Eye Mask for Travel Airplane Hotel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Transparent Composite Fiberglass Roof Tiles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blast Freezer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Two Layer License Plates with Wide Border Embossed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Locking Hinge Pin]

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Marathon Medal for Marathon Medallion]

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, Part I.Really Microfiber PVC Leather for Sofa Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting]

94V0 Circuit Board Mobile Phone PCB Motherboard, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, 175-184[12g Purple Small Church Activity Candle Holder Aluminum]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets]

Whitaker,J.R.(1963),Adhesive Powder for Skim Coat/Wall Putty HPMC. Analytical Chemistry35(12), 1950-1953[Kingdomway Brand Vitamin a 1000]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing MachineAuthor for correspondence