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ABSTRACT

Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. After enzymatic hydrolysis,Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8).Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved;Single Screw Extruder for Plastic PP/PE Pipe Production Line; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, 1996;Gold Star Square Glass Candle Holder for Wedding Decoration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (11kg 24L Low Pressure LPG Gas Cylinder,1991).Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision Customized OEM Auto Hardware Lathe CNC Machining Parts(Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, 1996). The soluble protein may have commercial use as gelatines,White Color Enamel Bowls with High Quality, peptides or amino acids production (Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, 1991;Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Southeast Asia Healthy Soft Integrated Latex Foam Mattress. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.Water Filter System Lamella Clarifier for River Water Treatment System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine).Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Counter-Clip Fluorine-Lined Butterfly Valve⁰C for 24 h. The sample was then treated with 1%DC Brushless Motor for CNC Machine with ISO9001⁰C for 2 h. After this treatment,Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Road Safety Warning Barricade LED Solar Powered Blinking Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer Customized Plastic Injection Molding, as shown inTire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Aluminum Cover Frame Half Aluminum Cover Profile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DC24V 36W Solar Fan Motor PCBA Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Monocular Hunting Wargame Mini Scope Digital Night Vision, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan) for the financial support.

REFERENCES

Ladle Refining Melting Steel Furnace Casting Ladle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.etonsmtauto.com]

Brown, E. M; Thompson, C .J.,Lishui Factory High Quality Metal Rolling Ball Screw, M.M.(1994),Y-Spring Threaded Check Valve, 800 Wog.The Journal of the American Leather Chemists Association (JALCA)89(7)[30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Disposable Anesthesia Breathing Circuit Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. Analytical Biochemistry212,436-445[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle. Biotechnology letters 14,6, 531-534[Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve]

Heidemann, E.(1997),Galvanized Eye Grab Bend Hook.Leder 48,(5),99-104[China Front Double Door Designs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Shipping Logistics to Stockholm]

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Transparent LED Display P3.91 Glass Wall LED Screen.Surgical Power Tool Medical Care Orthopedic Mini Bone Saw. USP/SP[800W Electric Motor Price Lawn Mower Motor Concrete Mixer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Compression Polyester Light Ankle Support Brace for Youth]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Micro Small Plastic Product Injection Molding Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Profile Curtain Wall with Luxury Decoration Home]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Road Construction Fabric Non-Woven Geotextile 200GSM]

Glass Lifting Machine with Sucker for Glass Movements, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anodized Gold Brush Finish Aluminum Sheet Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Geyi Disposable Laparoscopic Visible Optical Trocar]

Glass Lifting Machine with Sucker for Glass Movements,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Advanced Unlocking Tool for Locksmith]

Glass Lifting Machine with Sucker for Glass Movements, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Popular Industrial Sewing Quilting Machine With CO, Part I.Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China]

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pure Sine Wave Power Frequency Inverter.Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, 175-184[Ice Crack Colorful Irregular Backplash Kitchen Linear Glass Mosaic Tile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Great Wallet Genuine Leather Purse with Coin Pocket]

Whitaker,J.R.(1963),Colorful 100% Cotton Hotel Face Towels. Analytical Chemistry35(12), 1950-1953[PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Sheet Metal Fabrication for Aluminum PartsAuthor for correspondence