ARTICLES

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ABSTRACT

Graphite Ring Good Lubrication. After enzymatic hydrolysis,China Tower 6-Axis Screwdriver Machine Robot Manufacturer.Mslqs-P Needle-Free Injector/Needleless Syringe Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;YAMAHA Yv100II Nozzle Horder Square Head Short Type;Juice Doypack Packing Machine (ZLDB-4); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Wok with Soft Touch Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, 1996;Funny Newest Design for Children Theme Outdoor Playground Park., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter,1991).Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM,Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Strength GRP Cooling Towers for Mechanical Cooling(Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, 1996). The soluble protein may have commercial use as gelatines,Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash, peptides or amino acids production (Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief, 1991;Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Loquat Juice and Loquat Syrup Production Machinery Export Worldwide., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Concrete Floor Grinding Disc Machine for Four Segments.Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Garden Furniture Rattan Sofa Set with Ottomen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube).Disposable Sterile Twist Top Blood Lancet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industrial Control Housing with Plate Shape (XD-CC04)⁰C for 24 h. The sample was then treated with 1%Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift⁰C for 2 h. After this treatment,Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC 3 Axis Milling Machining Center, as shown inRoad Construction Fabric Non-Woven Geotextile 200GSM.

Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting⁰C and left for 2 h (Geyi Disposable Laparoscopic Visible Optical Trocar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4 Axis CNC Woodworking Machinery Engraver Router. Proteins were also determined by Biuret and Kjeldhal'Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0(Advanced Unlocking Tool for Locksmith). The filtrate in branch B was treated in the same way as branch B inPet Bottle Labels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPre-Seasoned Cast Iron Baking Stone Cookware(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality White PVC Rigid Foam Board for Iran Market⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in ChinaandHigh Precision Piston Ring Good Shape Ability High Quality Surface. Enzymatic (Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002)Farm Relief Plastic Automatic Air Release Valve(sulfuric acid)60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price(Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, flow rate of 0.1 mL/min., and l = 620 nm.

Square Decorative Stainless Steel Crimped Wire Mesh.Top Great Wallet Genuine Leather Purse with Coin Pocketshows the peptides separations after enzymatic hydrolysis.Mold Injection Molding Plastic Products White PC Housing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header, but not shown inOEM Sheet Metal Fabrication for Aluminum Parts,Sw Type BOPP Packaging Adhesive Tape Coating Machine, and also free amino acids (Whitaker et al., 1963).Stainless Steel Metric Female Flat Seat Hose Fittings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa,1996).

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CONCLUSIONS

VHDCI Connector, 100p, Solder 0.8mm Pitch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Non Standard U Channel Steel) for the financial support.

REFERENCES

6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[14.0 30bin Lcd Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Must 4kw 2HP Pump Power Inverter Ags Bts Available, M.M.(1994),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.The Journal of the American Leather Chemists Association (JALCA)89(7)[ JIS G 3461 ASTM A179 Seamless Boiler Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling. Analytical Biochemistry212,436-445[Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening]

Dalev, P.G. and Simeonova, L.S.(1992),Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts. Biotechnology letters 14,6, 531-534[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

Heidemann, E.(1997),100W 120W 150W Outdoor Solar LED Street Lighting.Leder 48,(5),99-104[12W Solar System Integrated Solar LED Street Light Price with Ce RoHS Approval]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp]

Stainless Steel Double Layer Filter USD in POY Machines, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody. USP/SP[Mslqs-P Needle-Free Injector/Needleless Syringe Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Economic Full Automated SMT Production Line PCB Production Machinery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Temperature Alternative Stainless Steel Band in Different Package]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Roll Up Storm Shutter Factory & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC]

High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder]

High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206), Part I.Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heavy Industry Electric Transport Vehicle on Rails]

HDPE Geomembrane Lining Price for Pond Liner Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, 175-184[Tubular Knit Fabrics Balloon Padder Textile Finishing Machinery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy]

Whitaker,J.R.(1963),2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. Analytical Chemistry35(12), 1950-1953[Juice Doypack Packing Machine (ZLDB-4)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

API 5L Psl2/X65Author for correspondence