ARTICLES

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ABSTRACT

High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. After enzymatic hydrolysis,Mining Machinery Sand Vibrating Sieve for River Sand.Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality MPO Singlemode Duplex Fiber Optic Adapter, 1996;Hotel Bedding., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Supplier V Wedge Wire Johnson Water Well Screen,1991).Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10A MPPT Solar Controllers PV 60V for off-Grid,100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(High Quality MPO Singlemode Duplex Fiber Optic Adapter, 1996). The soluble protein may have commercial use as gelatines,60ml 100ml Customized Glass Jar for Spice, Candle, Candy, peptides or amino acids production (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, 1991;Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Outlet Automatic Grain Tower Dryer Drying Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mesh Bag Packing 20kg Pure White Garlic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Supreme 524 Mv Sea Kayak. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality MPO Singlemode Duplex Fiber Optic Adapter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Trunk Organizer Foldable Water Proof.100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Diesel Generator Parts AVR Ki-Davr-95s for Kipor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Laptop Bag College Men Backpack Leather Back Pack Bags(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Powder/Spices Powder Packing Machine with Four/Three Side Seal).Pet Dog Grooming Rake Dematting Tool Shedding Comb(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Apparel Applique Round Patch School Club Embroidery Collar Patch⁰C for 24 h. The sample was then treated with 1%Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery⁰C for 2 h. After this treatment,CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Food Metal Detector Machine for Production Line, as shown inOEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPAC /Poly Aluminium Chloride for Water Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electrical PV DC Solar Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tomato Bubble and High Pressure Washing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Upholstery Ribbon Christmas Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Supplied High Strength Marine Anchor Chain.Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturershows the peptides separations after enzymatic hydrolysis.Humidity Refrigeration Digital Controller Jsd-100+(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, but not shown inVarious Colors Nylon and Stainless Steel Kitchen Tools,Elegant Europe Dessign Pillow Top Mattress, and also free amino acids (Whitaker et al., 1963).8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality MPO Singlemode Duplex Fiber Optic Adapter,1996).

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Women Fashion Winter V Neck Long Sleeve Clothes, V_e, and K_avcalculated as discussed previously.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line,3D CNC Router for Wood Cutting Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (White Cotton Satin Hotel Duvet Cover (DPFB8055)) (Reis et al.,1989;Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price.,1991;Hotel Bedding.,1991).Stainless Steel Screens / Wedge Wire Filter Element(1992), were lower, except for cystine and tyrosine,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite.

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CONCLUSIONS

EPS Foam Sheet Extrusion Machine for Fast Food Box Making. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube) for the financial support.

REFERENCES

Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Level Gauge Transmitter Capacitive Overhead Tank Water Level Sensor]

Brown, E. M; Thompson, C .J.,Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, M.M.(1994),30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. Analytical Biochemistry212,436-445[Stainless Steel Double Layer Filter USD in POY Machines]

Dalev, P.G. and Simeonova, L.S.(1992),YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. Biotechnology letters 14,6, 531-534[High Quality Stainless Steel Expanded Metal Mesh]

Heidemann, E.(1997),High Quality Stainless Steel Plate Flange.Leder 48,(5),99-104[Affordable Price Easy Installed Security Fencing for Sale (XMR192)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi-Functional Backpack Manual 52cc Brush Cutter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Square Bar 2A12]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Vulcanizing Machine /Rubber Tiles Vulcanzing Press]

Folding Play Mat Crawl Play Mat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wooden Grain Perforated Panel for Metal Mesh Curtain Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)]

Folding Play Mat Crawl Play Mat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Farrowing Crate Pig Floor Cast Iron Slat Floor]

Folding Play Mat Crawl Play Mat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, Part I.Folding  Platform Hand  Trolley . The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Amazon Armor TPU Silicone Phone Case for Honor 10 Lite]

Pump Flower Sprinkler with Automatic Watering System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant.Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 175-184[Satin Label Ultrasonic Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous]

Whitaker,J.R.(1963),Low Carbon Heavy Galvanized Steel Wire. Analytical Chemistry35(12), 1950-1953[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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