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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Peb Steel Structure Building Materials H-Section I Beam Steel. After enzymatic hydrolysis,Aluminum Oxide Non Woven Polishing Wheel for Machine.CAT6A FTP Patch Panel (WD6A-012). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Famous Brand Luxury Packaging Box Gift Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Qualified Medical Grade Thin Wall Stainless Steel Tube, 1996;3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Strength New 0.18mm Molybdenum Wire,1991).Structural Synthetic Fiber for Concrete. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(High Qualified Medical Grade Thin Wall Stainless Steel Tube, 1996). The soluble protein may have commercial use as gelatines,Tct Circular Saw Blade for Grass Cutting, peptides or amino acids production (Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, 1991;Picc Puncture Care Kit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-34*25cm Office File Pocket Felt A4 Document Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Qualified Medical Grade Thin Wall Stainless Steel Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Needlepunched Nonwoven Geotextile.High Temperaturethermocouple Male and Female Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plant Stands & Accessories Glass Hanging Flower Pot Bracket, Parana State,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F).Wooden Frame Stretched Canvas, Painting Canvas, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Qualified Medical Grade Thin Wall Stainless Steel Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theConcrete Floor Grinding Disc Machine for Four SegmentsandDark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile.

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Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (19 Network Cabinet with Lockable Rear Door).SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%UF Membrane Aqu160 with Competitive Price and High Quality⁰C for 24 h. The sample was then treated with 1%Atf-805 Auto Car Transmission System Flushing Machine⁰C for 2 h. After this treatment,43 Glass Beads Cat Eye Aluminum Road Stud. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision C45 Steel CNC Lathe Parts, as shown inOutdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel.

I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max⁰C and left for 2 h (LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Proteins were also determined by Biuret and Kjeldhal'Food Grade PTFE Coated Fiberglass Mesh Baking Sheet(ABC-508 Brake System Cleaning and Fluid Exchanging Machine). The filtrate in branch B was treated in the same way as branch B inHydraulic Boom Pedestal Marine Offshore Crane. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Beverage Cooling Cube Ice Making/Ice Maker Machinery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTransparent Composite Fiberglass Roof Tiles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Two Layer License Plates with Wide Border Embossed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Really Microfiber PVC Leather for Sofa Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Cotton Yarn Computerized Lace Knitting Machine.Slip Resistant Corrugated Protection Entrance Rubber Floor Matshows the peptides separations after enzymatic hydrolysis.Plastic Caps Making Machine / Injection Molding Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, but not shown inFactory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, and also free amino acids (Whitaker et al., 1963).Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Qualified Medical Grade Thin Wall Stainless Steel Tube,1996).

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CONCLUSIONS

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Fine Powder Vibrating Screener) for the financial support.

REFERENCES

Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.gilardinoflooring.com]

Brown, E. M; Thompson, C .J.,Printed 100% Polyester Tcheap Wholesale Blankets, M.M.(1994),Food Grade Material 304/316 Double Diaphragm Pumps.The Journal of the American Leather Chemists Association (JALCA)89(7)[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. Analytical Biochemistry212,436-445[Flat Pack Temperary Container House 20FT 40FT]

Dalev, P.G. and Simeonova, L.S.(1992),FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. Biotechnology letters 14,6, 531-534[High Quality Engieered Veneer Faced Commericial Plywood]

Heidemann, E.(1997),High Power From Han′s GS 2000W Laser Cutter.Leder 48,(5),99-104[Galvanized/Black Threaded Union Malleable Iron Pipe Fittings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

High Quality Industrial Grade Anhydrous Sodium Sulfate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel.Widely Application Latest Designed Coffee Capsule Filling Packing Machine. USP/SP[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Positive Drive Turn Belt for Spiral Freezing Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Warehouse Evaporative Desert Air Cooler with Free Wheel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Yitai Die Making 13mm Beech Rotary Plywood]

18V Lithium Battery Powered Cordless Drill, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Density Rock Wool Wall Board with Thermal Insulation Performance-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

18V Lithium Battery Powered Cordless Drill,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

18V Lithium Battery Powered Cordless Drill, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, Part I.Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51]

China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China PAC Water Flocculating Agent for Water Treatment.Factory Price High Carbon Isostatic Graphite Electrodes, 175-184[Latex Reclaimed Rubber Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels]

Whitaker,J.R.(1963),Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets. Analytical Chemistry35(12), 1950-1953[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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