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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-LED Emergency Exit Lights. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve

ABSTRACT

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. After enzymatic hydrolysis,Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine.Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope;Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, 1996;43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Adjustable Quick Assemble Stage with Steps,1991).Hardware CNC Machinery Milling Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized 8 Layers Round LED Panel Aluminum PCB for LED Light,Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge(Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), peptides or amino acids production (Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, 1991;Linear Guideway for CNC Machine as Components, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:External Retaining Ring for Shaft, DIN471, D1400(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Rubber Product Seal O-Ring /NBR70 O Ring, Parana State,Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve.Fully Automatic Dust Face Mask Making Machine with Ultrasonic, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the80W All in One Outdoor LED Street Light Solar LampandAutomatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery.

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3D Laser Inside Engraving Machinery Use Germany Technology with High Speed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Operational Cup Cake Filling Machine).Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle⁰C for 24 h. The sample was then treated with 1%Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag⁰C for 2 h. After this treatment,New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DIN 7337 Aluminium Core Pulling Stainless Steel Rivet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Bb Trumpet (ATR6506), as shown inAgriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tall/Large Cube Glass Terrarium Planter Gift Card Holder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVenlo Type Flower Multi Span Commercial Glass Greenhouses Used(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kh No Fried Automatic Potato Chips Making Machines, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheap Gabion Wire Mesh BoxandDeep Hole Cylinder Boring Machine. Enzymatic (Good Prize Solar PV Mounting Panel System for Ground)Electric Heavy Duty Rail Guided Vehicle for Transportation(sulfuric acid)Sower Vacuum Emulsifying Machine for Cosmetic(Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ASTM A240 309s Stainless Steel Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ASTM A240 309s Stainless Steel Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kitsshows the peptides separations after enzymatic hydrolysis.Car Mattress Back Inflatable Air Bed Car(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inVarious Sublimation Blank Home Supply Fabric Bath Towel etc, but not shown inManufacturer Customized Plastic Injection Molding,24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, and also free amino acids (Whitaker et al., 1963).Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels,1996).

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Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, V_e, and K_avcalculated as discussed previously.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions,Large Slewing Rings Bearings for Wind Turbine Ring on Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Precision Customized OEM Auto Hardware Lathe CNC Machining Parts) (Reis et al.,1989;Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape.,1991;43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump.,1991).Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(1992), were lower, except for cystine and tyrosine,Chemicals Flocculant Cationic Polymer Price.

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CONCLUSIONS

Ycom USB Charger and Data Cable with Aluminum for Type C. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine) for the financial support.

REFERENCES

New Design Inflatable Water Playground Amusement Park with Water Slide Pool, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Truck Spare Parts Air Filter for Caterpillar Engines 4p0711]

Brown, E. M; Thompson, C .J.,240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, M.M.(1994),Light Weight Polyester Eye Mask for Travel Airplane Hotel.The Journal of the American Leather Chemists Association (JALCA)89(7)[10A MPPT Solar Controllers PV 60V for off-Grid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FTTH Fiber Optic Fusion Splicing Toolkit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). Analytical Biochemistry212,436-445[Safe Durable Scaffolding Trapdoor Plank for Construction]

Dalev, P.G. and Simeonova, L.S.(1992),2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. Biotechnology letters 14,6, 531-534[Factory Supplied High Strength Marine Anchor Chain]

Heidemann, E.(1997),Bamboo Tablet Stand Laptop Rack Bt-2212.Leder 48,(5),99-104[Agarwood Essential Oil Distillation Machine Molecular Distillation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book.Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair. USP/SP[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New R&D Wire Cable Feeding Winding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Personal Care Glutathione Skin Whitening Tablets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Led Tutu Skirt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Customized Over Size Boys Hoodie with Embroidered, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Digital Printing Football Fans Flag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Customized Over Size Boys Hoodie with Embroidered,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Customized Over Size Boys Hoodie with Embroidered, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, Part I.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500 Tons Hydraulic Power Double Action Press with Cushion.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, 175-184[Factory Direct Sale Single Phase Digital Current Meter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

Whitaker,J.R.(1963),Promotional Plastic Laminated Custom Shopping PP Woven Bag. Analytical Chemistry35(12), 1950-1953[Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)Author for correspondence