ARTICLES

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ABSTRACT

Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. After enzymatic hydrolysis,OEM 15PCS Electric Face Makeup Brush with Ce Certificate.New Styles Fashion Erotic Women Underwear Sexy Lingerie. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale;Stand Hot and Cold Normal Water Dispenser with Refrigerator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Construction Welding Rods Electrode E6013 E7018 E7016, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, 1996;Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lydite Fence Reel Reel with Extension Arms for Farm Fencing,1991).1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized 3D Medal for Religious Medal Gift,Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dbry-320 Christmas Stickers Labels Printing Machine(High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, 1996). The soluble protein may have commercial use as gelatines,Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, peptides or amino acids production (Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, 1991;14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Bright Polished Mirror Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)Alcalase2.5 L DX,Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board).Molasses Fuel Ethanol Distillation Plant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pond Self Priming Booster Pressure Water Pump with Check Valve⁰C for 24 h. The sample was then treated with 1%Banded Type Tee Used on Oil⁰C for 2 h. After this treatment,20FT New Shipping Container 40FT Dry Cargo Container. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, as shown in3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card.

High Pressure Braided Rubber Oil Hose⁰C and left for 2 h (Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Monochrome Graphic Industrial Control LCD Display Module 128X64. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SMD LED Light-Operated USB Rechargeble Flashlight. Proteins were also determined by Biuret and Kjeldhal'Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden(China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump). The filtrate in branch B was treated in the same way as branch B inSquare Crossbody Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1jo4 Hydraulic Adaptors for Hose Fittings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese 1325 Ce Certificated CNC Engraving Router for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOil Heating Reactor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/All Kinds of Color Fashion Simple Stone Pendant Design Necklace, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in Chinaand48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Enzymatic (Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb)Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(sulfuric acid)Toilet Roll Tissue Wrapping Machine with Auto - Counting(High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Orange Monofilament Fishing Tackle Nylon Fish Net.Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flangeshows the peptides separations after enzymatic hydrolysis.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, but not shown in1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio,By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, and also free amino acids (Whitaker et al., 1963).Diameter 1300mm Lifting Electro Magnet for Cast Iron. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process,1996).

etc

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Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, V_e, and K_avcalculated as discussed previously.100%Polyester Spandex Hotel Chair Cover (DPH7022),Factory Supply Perforated Metal Mesh. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mass Stock Yl10.2 Carbide Rods for Milling 45HRC) (Reis et al.,1989;Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.,1991;Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular.,1991).CNC Turning Center, CNC Horizontal Turning Milling Machine(1992), were lower, except for cystine and tyrosine,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.

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CONCLUSIONS

Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W) for the financial support.

REFERENCES

Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.apolomed.com]

Brown, E. M; Thompson, C .J.,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, M.M.(1994),Factory Wholesale Zinc Plated Carbon Steel Hex Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. Analytical Biochemistry212,436-445[Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. Biotechnology letters 14,6, 531-534[Water Filter System Lamella Clarifier for River Water Treatment System]

Heidemann, E.(1997),Stainless Steel Cheese Making Machine Electric.Leder 48,(5),99-104[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Durable Aluminum Flag Pole with Dome Top Tangle Free Finial]

Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.100W Dimmable LED Corn Bulb with Meanwell Driver. USP/SP[Professional Motorcycle Reflector Injection Plastic Parts Mould Maker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Computer Plain Socks Knitting Machine with High Quality]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pet and Animal Food Extrusion Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Masonry Drill]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Factory Supply Design Cat Tree Pet Product]

Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hexagonal Wire Mesh Chicken Wire Mesh High Quality-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:V-32 New Special UPS Solar Battery Cabinet Box Case, Part I.4-14 Manual Staple Gun with Staples for Hand Tools. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine.Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, 175-184[Custom Logo Nylon Hook and Loop Cable Tie]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Products 16′ Perforated Flex Pipe]

Whitaker,J.R.(1963),Biomass Drum Wood Chipper with Competitive Price. Analytical Chemistry35(12), 1950-1953[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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