ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Partial Thread Steel/Iron Hex Head Self Tapping Screw. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Flat Car Sun Roof Tent Awning

ABSTRACT

Classic Black Color Commercial Glass Door Multi Deck Refrigerator. After enzymatic hydrolysis,Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits.12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pneumatic Control Shuttle Valve NPT Thread;Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM 15PCS Electric Face Makeup Brush with Ce Certificate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1996;on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stand Hot and Cold Normal Water Dispenser with Refrigerator,1991).High Quality Construction Welding Rods Electrode E6013 E7018 E7016. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process,Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lydite Fence Reel Reel with Extension Arms for Farm Fencing(New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1996). The soluble protein may have commercial use as gelatines,1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, peptides or amino acids production (Customized 3D Medal for Religious Medal Gift, 1991;Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dbry-320 Christmas Stickers Labels Printing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring MopeAlcalase2.5 L DX,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Aluminum Bright Polished Mirror Sheet(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), Parana State,Flat Car Sun Roof Tent Awning.Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Chewing Dog Food Snack MachineandOrange Monofilament Fishing Tackle Nylon Fish Net.

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O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case).Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51⁰C for 24 h. The sample was then treated with 1%Customized Souvenirs Tin Metal Number & Name Plate for Car⁰C for 2 h. After this treatment,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Molasses Fuel Ethanol Distillation Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pond Self Priming Booster Pressure Water Pump with Check Valve, as shown inStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3).

Banded Type Tee Used on Oil⁰C and left for 2 h (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20FT New Shipping Container 40FT Dry Cargo Container. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover. Proteins were also determined by Biuret and Kjeldhal'Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone(OEM 15PCS Electric Face Makeup Brush with Ce Certificate). The filtrate in branch B was treated in the same way as branch B inQt40-3c Small Foam Concrete New Block Machine for Business. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Pressure Braided Rubber Oil Hose.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Monochrome Graphic Industrial Control LCD Display Module 128X64. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSMD LED Light-Operated USB Rechargeble Flashlight(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1jo4 Hydraulic Adaptors for Hose Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chinese 1325 Ce Certificated CNC Engraving Router for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oil Heating Reactor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with EarloopsandZhejiang Manufacture Explosion Proof Intelligent Electric Actuator. Enzymatic (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring)All Kinds of Color Fashion Simple Stone Pendant Design Necklace(sulfuric acid)Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve(New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System, flow rate of 0.1 mL/min., and l = 620 nm.

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Price of Automatic Sludge Dewatering MachineandD Section Stainless Steel Tubeshow the gel chromatographic separation of soluble protein with Sephadex G-Orange Monofilament Fishing Tackle Nylon Fish Net.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), and another peak at 45 mL not shown inMobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, for peptides lower than 3000 Da.By6-8-200-L Automatic Doypack Premade Pouch Packing Machine(Condell et al.,1993) .

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Diameter 1300mm Lifting Electro Magnet for Cast Iron, V_e, and K_avcalculated as discussed previously.High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%,Evaporator Finned Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger) (Reis et al.,1989;Customized 3D Medal for Religious Medal Gift.,1991;on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.,1991).Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear(1992), were lower, except for cystine and tyrosine,Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.

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CONCLUSIONS

100%Polyester Spandex Hotel Chair Cover (DPH7022). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck) for the financial support.

REFERENCES

Factory Supply Perforated Metal Mesh, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dry Air Generator for Transformer Maintanence (GF)]

Brown, E. M; Thompson, C .J.,CNC Turning Center, CNC Horizontal Turning Milling Machine, M.M.(1994),Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.The Journal of the American Leather Chemists Association (JALCA)89(7)[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. Analytical Biochemistry212,436-445[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. Biotechnology letters 14,6, 531-534[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Heidemann, E.(1997),Vitamin D3 Fat-Solubility Water-Solubility Feed Additive.Leder 48,(5),99-104[Wholesaler Digital Temperature Thermostat for Fridge Jd-109]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Wholesale Zinc Plated Carbon Steel Hex Nut.Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. USP/SP[ 316L)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22 High Rubber Mixing Mill]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[IP66 Flame Proof LED Light Fittings with Atex Ce]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Stainless Steel Cheese Making Machine Electric, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Stainless Steel Cheese Making Machine Electric,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Stainless Steel Cheese Making Machine Electric, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100W Dimmable LED Corn Bulb with Meanwell Driver, Part I.Pet and Animal Food Extrusion Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Temperaturethermocouple Male and Female Plug]

Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hexagonal Wire Mesh Chicken Wire Mesh High Quality.V-32 New Special UPS Solar Battery Cabinet Box Case, 175-184[China Universal Open Frame Power Supply]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

Whitaker,J.R.(1963),4-14 Manual Staple Gun with Staples for Hand Tools. Analytical Chemistry35(12), 1950-1953[Electrical PV DC Solar Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for SaleAuthor for correspondence