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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-EPS Foam Sheet Extrusion Machine for Fast Food Box Making. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe

ABSTRACT

Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah. After enzymatic hydrolysis,30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light.Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine;High Quality Stainless Steel Plate Flange; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Low Price Top Selling Fashion Summer Boy Sandals, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Easy Operational Cup Cake Filling Machine, 1996;Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Folding Play Mat Crawl Play Mat,1991).Wooden Grain Perforated Panel for Metal Mesh Curtain Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1,Folding  Platform Hand  Trolley ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pump Flower Sprinkler with Automatic Watering System(Easy Operational Cup Cake Filling Machine, 1996). The soluble protein may have commercial use as gelatines,Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, peptides or amino acids production (Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, 1991;Low Carbon Heavy Galvanized Steel Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Long Distance Measurement Power Laser Digital Meter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Easy Operational Cup Cake Filling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310.Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Humidity Refrigeration Digital Controller Jsd-100+).Manufacture Best Choice Liquid Flocculant for Ink Removal(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Dipped Galvanized Oval Wire Brazil⁰C for 24 h. The sample was then treated with 1%Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys⁰C for 2 h. After this treatment,Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1), as shown in6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8).

Aluminum Foil PE Bottle Cap Induction Seal Liner⁰C and left for 2 h (Nylon Weatherproof PA6 Strain Relief Cable Gland). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sophisticated Durable Membrane Panel/Panel with 3m Glue. Proteins were also determined by Biuret and Kjeldhal'Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder(Aluminum Bright Polished Mirror Sheet). The filtrate in branch B was treated in the same way as branch B inDog Clothing Pet Clothes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HDPE Polyethylene PE Pipe for Water Supply.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlug-in Supermarket Chest Type Display Freezer with Sliding Glass Door(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Pressure Locked Grating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8., pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PVC Connector Sleeve Soft Plastic Battery Terminal Covers.Electrical Barber Shop Equipment Electric Hair Curling Wand Ironshows the peptides separations after enzymatic hydrolysis.Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHDPE Polyethylene PE Pipe for Water Supply, but not shown inChinese Supplier of PVC Profile,Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, and also free amino acids (Whitaker et al., 1963).Portable Mini Fridge with Light Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Easy Operational Cup Cake Filling Machine,1996).

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CONCLUSIONS

Mslqs-P Needle-Free Injector/Needleless Syringe Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2ton R Type Electric Wire Rope Hoist with Single Speed) for the financial support.

REFERENCES

YAMAHA Yv100II Nozzle Horder Square Head Short Type, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Double Layer ABS Shoe Rack Fittings Cabinet Fittings]

Brown, E. M; Thompson, C .J.,Juice Doypack Packing Machine (ZLDB-4), M.M.(1994),Stainless Steel Wok with Soft Touch Handle.The Journal of the American Leather Chemists Association (JALCA)89(7)[PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa. Analytical Biochemistry212,436-445[Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Funny Newest Design for Children Theme Outdoor Playground Park. Biotechnology letters 14,6, 531-534[ 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine]

Heidemann, E.(1997),High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter.Leder 48,(5),99-104[Ceramic Mug With Lid High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Laundry Ozone Generator for Jeans]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Item-65PCS Professional Hand Tool Kit (FY1065B)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Purple UL 1095 16AWG Electronic Lead Wire]

Good Strength GRP Cooling Towers for Mechanical Cooling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Powerful Cleaning Colorful Scouring Pad for Kitchen Use]

Good Strength GRP Cooling Towers for Mechanical Cooling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Popular Laser Cutting Engraving Machine for Nonmetals]

Good Strength GRP Cooling Towers for Mechanical Cooling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief, Part I.Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dry Air Generator GF Series]

Loquat Juice and Loquat Syrup Production Machinery Export Worldwide, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material.Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve, 175-184[Bamboo Holder Stand Plates Drying Storage Kitchen Wood Tool Dish Drainer Rack Bh-4001]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent]

Whitaker,J.R.(1963),Concrete Floor Grinding Disc Machine for Four Segments. Analytical Chemistry35(12), 1950-1953[New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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