ARTICLES

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ABSTRACT

Printed 100% Polyester Tcheap Wholesale Blankets. After enzymatic hydrolysis,Food Grade Material 304/316 Double Diaphragm Pumps.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Widely Application Latest Designed Coffee Capsule Filling Packing Machine, 1996;Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (18V Lithium Battery Powered Cordless Drill,1991).High Density Rock Wool Wall Board with Thermal Insulation Performance. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh,Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery(Widely Application Latest Designed Coffee Capsule Filling Packing Machine, 1996). The soluble protein may have commercial use as gelatines,China PAC Water Flocculating Agent for Water Treatment, peptides or amino acids production (Factory Price High Carbon Isostatic Graphite Electrodes, 1991;Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Widely Application Latest Designed Coffee Capsule Filling Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Powder/Spices Powder Packing Machine with Four/Three Side Seal.Black Phosphated Bugle Head Csk Head Drywall Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

China Safety Long Boom Mini Excavator Track Chain Hydraulic PumpAlcalase2.5 L DX,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-6 Inch Plastic Breathing Cap for The IBC Valve); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9).Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars⁰C for 24 h. The sample was then treated with 1%Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer⁰C for 2 h. After this treatment,Freshwater Pearl Butterfly Flower Bride Hairpin. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Bubble Insulation Thermal Building Insulation Material's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, as shown inLady Kids Child Yoga Clothes Gym Fitness Sports Wear.

Rechargeable Battery Operated Cooling Folding Electric Fan⁰C and left for 2 h (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,21.5′′ Wall Mount Display Capacitive Touch Monitor. Proteins were also determined by Biuret and Kjeldhal'Pagoda Gazebo Tent in Guangzhou for Sale(Different Size of Ring NdFeB Magnet). The filtrate in branch B was treated in the same way as branch B in18mm Poplar Core Plywood. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack Small Fridge for Showcase(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Speed Roll Plastic T-Shirt Bag Making Machine.Slewing Gear for Tower Crane Slewing Winch Reducershows the peptides separations after enzymatic hydrolysis.Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, but not shown inMechanical Seal 101 Type for Pump and Auto,Wholesale Black Galvanized Head Stainless Steel Screw, and also free amino acids (Whitaker et al., 1963).PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Widely Application Latest Designed Coffee Capsule Filling Packing Machine,1996).

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CONCLUSIONS

Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6 Inch Plastic Breathing Cap for The IBC Valve) for the financial support.

REFERENCES

6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Duplex 2507 Stainless Steel Sheet]

Brown, E. M; Thompson, C .J.,High Level Application Waterproof Plastic Box, M.M.(1994),Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 100p]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Analytical Biochemistry212,436-445[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

Dalev, P.G. and Simeonova, L.S.(1992),Water Slide for Indoor Play Structure. Biotechnology letters 14,6, 531-534[Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale]

Heidemann, E.(1997),Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder.Leder 48,(5),99-104[Electric Food Kiosk]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

CNC Machining Service High Precision CNC Mechanical Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1220*2440mm Concrete Formwork black film faced plywood.Washami Teeth Care for Whitening Cool Mint Toothpaste. USP/SP[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1600x Biological Microscope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring]

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SHXJ1500 CNC Diamond Wire Stone Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap]

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Machining Non-Standard Hardware Parts]

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, Part I.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yyh Series One Dimension Tumbling Mixer Mixing Machine.ODU 14 Pin Industrial Circular Connector, 175-184[China Air Filter For Air Compressor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Whitaker,J.R.(1963),Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. Analytical Chemistry35(12), 1950-1953[Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Southeast Asia Healthy Soft Integrated Latex Foam MattressAuthor for correspondence