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ABSTRACT

20FT New Shipping Container 40FT Dry Cargo Container. After enzymatic hydrolysis,High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Pressure Braided Rubber Oil Hose;Monochrome Graphic Industrial Control LCD Display Module 128X64; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SMD LED Light-Operated USB Rechargeble Flashlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 1996;1jo4 Hydraulic Adaptors for Hose Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chinese 1325 Ce Certificated CNC Engraving Router for Sale,1991).Oil Heating Reactor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,All Kinds of Color Fashion Simple Stone Pendant Design Necklace(Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 1996). The soluble protein may have commercial use as gelatines,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve, peptides or amino acids production (48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System, 1991;Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Toilet Roll Tissue Wrapping Machine with Auto - Counting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Orange Monofilament Fishing Tackle Nylon Fish Net.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:By6-8-200-L Automatic Doypack Premade Pouch Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Diameter 1300mm Lifting Electro Magnet for Cast IronAlcalase2.5 L DX,High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Evaporator Finned Tube); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, Parana State,Banded Type Tee Used on Oil.100%Polyester Spandex Hotel Chair Cover (DPH7022), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePicosecond Laser Tattoo Removal Skin Care Medical Instrumentand Machine for Making.

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Factory Supply Perforated Metal Mesh(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steam Eye Mask Heating One Eye Mask for Sleeping).CNC Turning Center, CNC Horizontal Turning Milling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16⁰C for 24 h. The sample was then treated with 1%Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use⁰C for 2 h. After this treatment,Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vitamin D3 Fat-Solubility Water-Solubility Feed Additive's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Wholesale Zinc Plated Carbon Steel Hex Nut, as shown inHigh Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.

Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern⁰C and left for 2 h (Banded Type Tee Used on Oil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Cheese Making Machine Electric. Proteins were also determined by Biuret and Kjeldhal'Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts(Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building). The filtrate in branch B was treated in the same way as branch B in450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100W Dimmable LED Corn Bulb with Meanwell Driver.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet and Animal Food Extrusion Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.V-32 New Special UPS Solar Battery Cabinet Box Case⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4-14 Manual Staple Gun with Staples for Hand Tools, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pet Anti-Static Needled MatandFashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine. Enzymatic (Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness)Biomass Drum Wood Chipper with Competitive Price(sulfuric acid)High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags(Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Forged Steel Pressure Sealed Bonnet Globe Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Forged Steel Pressure Sealed Bonnet Globe Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic Injection Moulding Machine Supplier / Factory /Manufacturer.Black Small Fridge for Showcaseshows the peptides separations after enzymatic hydrolysis.Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTall/Large Cube Glass Terrarium Planter Gift Card Holder, but not shown in200W UFO LED High Bay Light Fixture for Interior Exterior Applications,15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, and also free amino acids (Whitaker et al., 1963).Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden,1996).

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Beverage Cooling Cube Ice Making/Ice Maker Machineryand30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Trapsshow the gel chromatographic separation of soluble protein with Sephadex G-80W All in One Outdoor LED Street Light Solar Lamp.Hot Sale Safety Vinyl PVC Flooring, and another peak at 45 mL not shown inMagic Hair Twist   Curl Sponge Brush, for peptides lower than 3000 Da.Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation(Condell et al.,1993) .

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CONCLUSIONS

XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Evaporator Finned Tube) for the financial support.

REFERENCES

Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[240ml 8oz Honey Pickles Glass Jar and Twist off Cap]

Brown, E. M; Thompson, C .J.,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, M.M.(1994),SUS316 Hexagon Socket Set Screws with Cone Point, DIN914.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Precision Machining Auto Parts Metal Contact]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Funny Newest Design for Children Theme Outdoor Playground Park]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale. Analytical Biochemistry212,436-445[Braiding Machine for Weaving Flexible Metal Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor. Biotechnology letters 14,6, 531-534[ Nc Studio Control]

Heidemann, E.(1997),Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC.Leder 48,(5),99-104[Made in China High Quality HS-600 Stainless Cable Ties Tool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Molasses Fuel Ethanol Distillation Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Explosion-Proof Housing Sealing Dispensing Machine.Tube Mills for Thin Wall Tubing. USP/SP[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Metric Female Flat Seat Hose Fittings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Fabric Rapid Roller Shutter for Clearn Room]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hotel Towel Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Car Badge Custom Emblem Auto Logo Sign for Advertising, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Car Badge Custom Emblem Auto Logo Sign for Advertising,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

Car Badge Custom Emblem Auto Logo Sign for Advertising, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), Part I.Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

Rear Wheel Exchangeable Aluminum Manual Wheelchair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow.Graphic 128X128 Dots Cog LCD, 175-184[UF Tubular inorganic Ultrafiltration porous Tube Alumina Ceramic Membrane Water Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type]

Whitaker,J.R.(1963),Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. Analytical Chemistry35(12), 1950-1953[Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Commercial Use Click Lock Standard Spc Vinyl Plank FlooringAuthor for correspondence