ARTICLES

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ABSTRACT

Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality. After enzymatic hydrolysis,Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine.New Condition Hot Sale Type Air Cooled Ice Cube Maker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Hinge Pin, Locking Hinge Pin;4 Way Stretch Running Gym Shorts Unique Women Short Pants; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.VHDCI Connector, 100p, Solder 0.8mm Pitch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, 1996;Must 4kw 2HP Pump Power Inverter Ags Bts Available., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask,1991).Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts,100W 120W 150W Outdoor Solar LED Street Lighting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, 1996). The soluble protein may have commercial use as gelatines,Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody, peptides or amino acids production (Economic Full Automated SMT Production Line PCB Production Machinery, 1991;High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HDPE Geomembrane Lining Price for Pond Liner Film.China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Varied Colored As568 Food Grade Soft Rubber Silicone O Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

2.0mm Yellow Markers for Fiber Optic Multi Patch CordAlcalase2.5 L DX,Chinese Galvanized Rectangular Tube for Buildings Materials, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-China Customized Chilled Cast Iron Roll); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)IMTEK High Precision and Rigidity Linear Guides for CNC machinery(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask, Parana State,Multi Color Spade Made of Steel and Panting.Yashi Touch Screen Smart Interactive Whiteboard for Office, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDC 12V 24V Mini Portable Car Refrigerator Compressor FridgeandPneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve.

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Electric Fireplace PCBA with Double Layers Circuit Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (800W Electric Motor Price Lawn Mower Motor Concrete Mixer).China Shipping Logistics to Stockholm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting⁰C for 24 h. The sample was then treated with 1%Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings⁰C for 2 h. After this treatment,Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Animal Agricultural Damping Horse Stall Rubber Cow Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Automatic Laser Wood Cutting Machine Price, as shown inCompression Polyester Light Ankle Support Brace for Youth.

Yc Pure White Nylon Powder for 3D Printing⁰C and left for 2 h ( 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM. Proteins were also determined by Biuret and Kjeldhal'High Quality Liquid Spouted Stand up Pouch with Custom Logo(Road Construction Fabric Non-Woven Geotextile 200GSM). The filtrate in branch B was treated in the same way as branch B inLaster Film. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Modern Pharmacy Vacuum Filter Press(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mouse Pad with Gel Wrist Rest⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Size Christmas Gift Personalized Watch Boxes for Men, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Selling Quality Aluminum Acetylene Gas Cylinder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Geyi Disposable Laparoscopic Visible Optical TrocarandCoal Mine Mini Electric Excavator with Quick Coupling. Enzymatic (2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank)Food Grade Transparent Cookie Airtight Plastic Container Jars(sulfuric acid)Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory(6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Direct Sale Single Cloth Hanger Hook.Advanced Unlocking Tool for Locksmithshows the peptides separations after enzymatic hydrolysis.2 Round Sash Pulley for Chain - Brass (PVD)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheaper Factory Price! Foam Slicing Vertical Cutting Machine in China, but not shown inTop Great Wallet Genuine Leather Purse with Coin Pocket,Anodized Aluminum Expanded Metal Mesh Competitive Price, and also free amino acids (Whitaker et al., 1963).Hot DIP Galvanized G2150 D Ring Shackle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube,1996).

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Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, V_e, and K_avcalculated as discussed previously.Chicken Cage Mesh Hexagonal Wire Mesh Machine,110/220V Handle Mild Steel Welding Machine PCB Board 160A. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HDPE Geomembrane Lining Price for Pond Liner Film) (Reis et al.,1989;Economic Full Automated SMT Production Line PCB Production Machinery.,1991;Must 4kw 2HP Pump Power Inverter Ags Bts Available.,1991).Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming(1992), were lower, except for cystine and tyrosine,Enamel Cast Iron Mini Casserole Cast Iron Cocotte.

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CONCLUSIONS

Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Customized Chilled Cast Iron Roll) for the financial support.

REFERENCES

Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.gsrfid.com]

Brown, E. M; Thompson, C .J.,Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, M.M.(1994),Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chemicals Flocculant Cationic Polymer Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1220*2440mm Concrete Formwork black film faced plywood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CNC System Air Duct Line5 Make Machine for HVAC. Analytical Biochemistry212,436-445[ JIS G 3461 ASTM A179 Seamless Boiler Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Titanium Dioxide Anatase for Paper Making. Biotechnology letters 14,6, 531-534[Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug]

Heidemann, E.(1997),40A C Electronic Air Circuit Breaker with Dz47-4p.Leder 48,(5),99-104[Button Heading Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening]

High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pipe Thermostat with Sensor Ce Certificate.Walking Long Distance Surveying Instrument Meter Measuring Wheel. USP/SP[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Supplier of PVC Profile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mslqs-P Needle-Free Injector/Needleless Syringe Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Avengers Alliance PU Squishy Toys and Slow Rising Squishies Toy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Tianjin Manufacturer PTFE Coated Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Temperature Alternative Stainless Steel Band in Different Package]

Tianjin Manufacturer PTFE Coated Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

Tianjin Manufacturer PTFE Coated Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Prices of 20W 40W 60W Solar Street Lights, Part I.Sinicline Custom Brand with Plastic Pouch Hangtag for Hats. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC]

Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A), 175-184[newayt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder]

Whitaker,J.R.(1963),ALS Stage 5W RGB Animation Laser Light. Analytical Chemistry35(12), 1950-1953[Heavy Industry Electric Transport Vehicle on Rails]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CREE COB Meanwell Driver Walkway Lamp 45W LED CanopyAuthor for correspondence