ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,The Most Competitive Sodium Gluconate for Water Reducer

ABSTRACT

Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. After enzymatic hydrolysis,Electric Magic Hot Hair Styling Curling Iron with Brushes.Melt Pressure Transducer (PT133). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe;Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, 1996;Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord,1991).Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial,Customized Marathon Medal for Marathon Medallion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Price of a Retail Supermarket Push Cart for Sale(Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, 1996). The soluble protein may have commercial use as gelatines,Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, peptides or amino acids production (Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 1991;Hotel Steel Digital Room Safes with Large LED Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Biogas/Methane Purification System/Desulfurization/Scrubber Tower.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Popular Custom School Uniform Woven Badge Patch with Merrow Border.1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Electrical Stationary Hoist Tower Crane 500kg MotorAlcalase2.5 L DX,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Flow Wrapper/ Bakery Moon Cake Packing Machine, Parana State,The Most Competitive Sodium Gluconate for Water Reducer.Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlow Wrapper/ Bakery Moon Cake Packing MachineandThe Most Competitive Sodium Gluconate for Water Reducer.

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Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China PAC Water Flocculating Agent for Water Treatment).Reusable Metal Stainless Steel Drinking Straws(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Designed Steel Frame Factory Building⁰C for 24 h. The sample was then treated with 1%Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate⁰C for 2 h. After this treatment,0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Scouring Pads Making Machine for Brush King's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, as shown inPicosecond Laser Tattoo Removal Skin Care Medical Instrument.

Glass Mix Stone Mosaic Tiles⁰C and left for 2 h ( Machine for Making). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank. Proteins were also determined by Biuret and Kjeldhal'Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net(High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card). The filtrate in branch B was treated in the same way as branch B in1m Walk-Behind Concrete Power Trowel Machine with Honda Gx160. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Used/Second Hand 120mpm Continous Galvanizing Line/Cgi.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRacing Preformance PTFE Teflon Alloy Fuel Adapter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Stainless Steel Expanded Metal Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Popular Lovely Animal Design Inflatable Slide for Kids, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Steam Eye Mask Heating One Eye Mask for SleepingandGYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable. Enzymatic (Hairs Comb for Pet Dog Cats)Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall(sulfuric acid)Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine(Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Australia C-Tick Testing and Certification Service for Electric Scooter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Australia C-Tick Testing and Certification Service for Electric Scooter, flow rate of 0.1 mL/min., and l = 620 nm.

STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfengshows the peptides separations after enzymatic hydrolysis.35 Degree Escalator with Auto Start Function in Parallel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBanded Type Tee Used on Oil, but not shown inCheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, and also free amino acids (Whitaker et al., 1963).Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game,1996).

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Black Small Fridge for ShowcaseandTall/Large Cube Glass Terrarium Planter Gift Card Holdershow the gel chromatographic separation of soluble protein with Sephadex G-Roller Wedge Screen Cylinder.Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, and another peak at 45 mL not shown in200W UFO LED High Bay Light Fixture for Interior Exterior Applications, for peptides lower than 3000 Da.Push Pull Small Cable to Cable Connector(Condell et al.,1993) .

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Stainless Steel Seamless Pipe ASTM A312 TP304 316, V_e, and K_avcalculated as discussed previously.Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Beverage Cooling Cube Ice Making/Ice Maker Machinery) (Reis et al.,1989;Lady Kids Child Yoga Clothes Gym Fitness Sports Wear.,1991;Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.,1991).Big Chocolate Storage Holding Tank, Chocolate Buffer Tank(1992), were lower, except for cystine and tyrosine,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.

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CONCLUSIONS

Asco 353 Series 3/4 Pulse Valve Solenoid Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange) for the financial support.

REFERENCES

China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Paper Laminated Sheet]

Brown, E. M; Thompson, C .J.,Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, M.M.(1994),Purple UL 1095 16AWG Electronic Lead Wire.The Journal of the American Leather Chemists Association (JALCA)89(7)[Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brushed Anodizing Aluminum Extrusion Profile for LED. Analytical Biochemistry212,436-445[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. Biotechnology letters 14,6, 531-534[Black Red Open Net Spandex Nylon Sexy Lingerie]

Heidemann, E.(1997),S235jo S235jr Steel Pipe Tube Shs Rhs.Leder 48,(5),99-104[m.jiaguanadulttoys.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Machining Auto Parts Metal Contact]

China Bearing with Housing Bearing / Pillow Block Bearing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing.Pre-Seasoned Cast Iron Mini Oval Casserole Pot. USP/SP[Funny Newest Design for Children Theme Outdoor Playground Park]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Braiding Machine for Weaving Flexible Metal Hose]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Square Wire Mesh Netting with SGS: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Nc Studio Control]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1 4 Zip Fleece Pullover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Metric Female Flat Seat Hose Fittings]

Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Garden Save Energy with Solar Flame Wall Light, Part I.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Fabric Rapid Roller Shutter for Clearn Room]

Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length.Non Stick Silicone Coated Fiberglass Cloth, 175-184[High Quality Betaine Anhydrous Hydrochloride Betaine HCl]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Whitaker,J.R.(1963),Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. Analytical Chemistry35(12), 1950-1953[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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