ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence

ABSTRACT

6 Inch Plastic Breathing Cap for The IBC Valve. After enzymatic hydrolysis,Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree;OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 1996;Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Freshwater Pearl Butterfly Flower Bride Hairpin,1991).Air Bubble Insulation Thermal Building Insulation Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh,Rechargeable Battery Operated Cooling Folding Electric Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve(Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 1996). The soluble protein may have commercial use as gelatines,21.5′′ Wall Mount Display Capacitive Touch Monitor, peptides or amino acids production (Pagoda Gazebo Tent in Guangzhou for Sale, 1991;Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Small Fridge for Showcase.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, Parana State,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence.High Speed Roll Plastic T-Shirt Bag Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGym Fitness Equipment Adjustable Weight BenchandHot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb.

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Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Reusable Metal Stainless Steel Drinking Straws).Wholesale Black Galvanized Head Stainless Steel Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine⁰C for 24 h. The sample was then treated with 1%45*60cm Anti-Fatigue ESD Rubber Floor Mat⁰C for 2 h. After this treatment,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vinyl Glove in Work & Labor Glove's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Energy Needed Compact Flour Mill Plant for Milling Wheat, as shown inFlb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

Nylon Weatherproof PA6 Strain Relief Cable Gland⁰C and left for 2 h ( D1400). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Steel Roller Embossed Paper 2019 New Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. Proteins were also determined by Biuret and Kjeldhal'Factory Price DIN5685 Galvanized Marine Anchor Link Chain(Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)). The filtrate in branch B was treated in the same way as branch B inCabinet Lock Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Level Application Waterproof Plastic Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Water Slide for Indoor Play Structure, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Pressure Fitting Quick Coupling Hydraulic Plugand1220*2440mm Concrete Formwork black film faced plywood. Enzymatic (Washami Teeth Care for Whitening Cool Mint Toothpaste)Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(sulfuric acid)Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine(Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%SHXJ1500 CNC Diamond Wire Stone Cutting Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%SHXJ1500 CNC Diamond Wire Stone Cutting Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensorshows the peptides separations after enzymatic hydrolysis.2019 Foam Concrete Machine/Foamed Concrete Block Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Hv / Coating Material Grade CMC Medium Viscosity, but not shown inHigh Efficiency Fine Powder Vibrating Screener,Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, and also free amino acids (Whitaker et al., 1963).Yyh Series One Dimension Tumbling Mixer Mixing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars,1996).

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OEM Double Sided Rigid PCB Circuit Board Manufacturer, V_e, and K_avcalculated as discussed previously.Robot of World Robotics Competition,Professional Rope/Cable/Wire Braiding Machine with Factory Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Price High Carbon Isostatic Graphite Electrodes) (Reis et al.,1989;Pagoda Gazebo Tent in Guangzhou for Sale.,1991;Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.,1991).Aluminum PCB Circuit Board SMD PCBA LED PCB(1992), were lower, except for cystine and tyrosine,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).

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CONCLUSIONS

Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood) for the financial support.

REFERENCES

China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Selling for Plastic Buffer For Sliding Door - AD3650 - Zhuohui]

Brown, E. M; Thompson, C .J.,Concrete Cure Spray Concrete Curing Agent, M.M.(1994),Automatic Chewing Dog Food Snack Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Non-Expensive Cracking Agent]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Venlo Type Flower Multi Span Commercial Glass Greenhouses Used]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Ceramic Shot Glass for Sublimation Printing - 1.5oz. Analytical Biochemistry212,436-445[What′s Kind of Machine for Making Organic Fertilizer Granules]

Dalev, P.G. and Simeonova, L.S.(1992),Promotional Gifts Custom Printed Silicone Wristband/Bracelet. Biotechnology letters 14,6, 531-534[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Heidemann, E.(1997),Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door.Leder 48,(5),99-104[www.changshanfabric.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Evaporator Finned Tube]

Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton.Farrowing Crate Pig Floor Cast Iron Slat Floor. USP/SP[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cog Graphic FPC LCD Display Module]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gift Boxes For Wine Glasses]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

High Quality Aluminum Heat Sink for Electronics Computer Electric, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

High Quality Aluminum Heat Sink for Electronics Computer Electric,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

High Quality Aluminum Heat Sink for Electronics Computer Electric, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Surprise! Range Hood Free! ! ! Juice Cart, Part I.Steam Eye Mask Heating One Eye Mask for Sleeping. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Automatic French Fries Frying Machine with Low Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, 175-184[water proof CPE/PE disposable mattress cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Whitaker,J.R.(1963),Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Analytical Chemistry35(12), 1950-1953[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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