ARTICLES

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ABSTRACT

Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW). After enzymatic hydrolysis,Food Machine Cube Ice Machine for Commercial Use.Christmas Decoration Rose Flower LED Light String. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;D Section Stainless Steel Tube;Mint Flavor 50ml Home Foam Toothpaste; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dxz Limit Switch Tower Crane Spare Parts, 1996;OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High-End Business Magnet Leather Ring Binder Notebook,1991).Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,Eyes- Caring LED Night Light for Nursing Reading with Soft Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Bearing with Housing Bearing / Pillow Block Bearing(Dxz Limit Switch Tower Crane Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, peptides or amino acids production (The Most Competitive Sodium Gluconate for Water Reducer, 1991;Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Magic Hot Hair Styling Curling Iron with Brushes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Melt Pressure Transducer (PT133).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dxz Limit Switch Tower Crane Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Marathon Medal for Marathon Medallion, Parana State,360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1).Price of a Retail Supermarket Push Cart for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dxz Limit Switch Tower Crane Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLydite Fence Reel Reel with Extension Arms for Farm FencingandFire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket.

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Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Souvenirs Tin Metal Number & Name Plate for Car).Lady Kids Child Yoga Clothes Gym Fitness Sports Wear(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hotel Steel Digital Room Safes with Large LED Display⁰C for 24 h. The sample was then treated with 1%Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture⁰C for 2 h. After this treatment,Biogas/Methane Purification System/Desulfurization/Scrubber Tower. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Popular Custom School Uniform Woven Badge Patch with Merrow Border, as shown inXPS Extruded Polystyrene Foam Board.

1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor⁰C and left for 2 h (Custom Rubber Product Seal O-Ring /NBR70 O Ring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrical Stationary Hoist Tower Crane 500kg Motor. Proteins were also determined by Biuret and Kjeldhal'Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(Modern 4 Person Workstation with Computer Desk Modular Office Furniture). The filtrate in branch B was treated in the same way as branch B inBeacon Technology Suppliers/Manufacturers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlow Wrapper/ Bakery Moon Cake Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Reusable Metal Stainless Steel Drinking Straws, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Designed Steel Frame Factory Building, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign BoardandDetergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. Enzymatic (0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table)Scouring Pads Making Machine for Brush King(sulfuric acid)Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor(Dxz Limit Switch Tower Crane Spare Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Glass Mix Stone Mosaic Tiles, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Glass Mix Stone Mosaic Tiles, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck) for the financial support.

REFERENCES

Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.tstartincan.net]

Brown, E. M; Thompson, C .J.,Australia C-Tick Testing and Certification Service for Electric Scooter, M.M.(1994),STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[No Lead Bronze Flange Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-35 Degree Escalator with Auto Start Function in Parallel. Analytical Biochemistry212,436-445[1220 Fork Length 3-Wheel Electric Forklift Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings. Biotechnology letters 14,6, 531-534[ Miniature Ball Bearing (616)]

Heidemann, E.(1997),Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h.Leder 48,(5),99-104[Factory Supply Dongfeng 16cbm Water Truck Dongfeng 6X4 Water Sprinkler Truck]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sell Mechanical Bar Screen for Water Treatment]

Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Roller Wedge Screen Cylinder.Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. USP/SP[Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Push Pull Small Cable to Cable Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Worm Gear Operation Wafer Lined F3 Butterfly Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom OEM Small Tn Htn Stn FSTN Full Viewing Angle 7 Segment Indicator LCD Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display]

Stainless Steel Seamless Pipe ASTM A312 TP304 316, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Jar Opener]

Stainless Steel Seamless Pipe ASTM A312 TP304 316,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polyglycolic Acid Absorable Suture]

Stainless Steel Seamless Pipe ASTM A312 TP304 316, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, Part I.Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4 Axis CNC Woodworking Machinery Engraver Router]

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Asco 353 Series 3/4 Pulse Valve Solenoid Valve.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, 175-184[Medical Devices]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light]

Whitaker,J.R.(1963),Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. Analytical Chemistry35(12), 1950-1953[Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

9004-78-8.Author for correspondence