ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine

ABSTRACT

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine. After enzymatic hydrolysis,Tct Circular Saw Blade for Grass Cutting.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Picc Puncture Care Kit;Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (34*25cm Office File Pocket Felt A4 Document Bag, 1996;PP Needlepunched Nonwoven Geotextile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Temperaturethermocouple Male and Female Plug,1991).34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Price Custom Embroidery Logo Hotel Slippers,Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Efficiency Fine Powder Vibrating Screener(34*25cm Office File Pocket Felt A4 Document Bag, 1996). The soluble protein may have commercial use as gelatines,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, peptides or amino acids production (Plant Stands & Accessories Glass Hanging Flower Pot Bracket, 1991;Wooden Frame Stretched Canvas, Painting Canvas, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UF Membrane Aqu160 with Competitive Price and High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (34*25cm Office File Pocket Felt A4 Document Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Atf-805 Auto Car Transmission System Flushing Machine.43 Glass Beads Cat Eye Aluminum Road Stud-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, Parana State,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine.Beverage Cooling Cube Ice Making/Ice Maker Machinery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (34*25cm Office File Pocket Felt A4 Document Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the22 High Rubber Mixing MillandProfessional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner.

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Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1).Transparent Composite Fiberglass Roof Tiles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125⁰C for 24 h. The sample was then treated with 1%Two Layer License Plates with Wide Border Embossed⁰C for 2 h. After this treatment,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Really Microfiber PVC Leather for Sofa Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)94V0 Circuit Board Mobile Phone PCB Motherboard, as shown inHigh Temperaturethermocouple Male and Female Plug.

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers⁰C and left for 2 h (New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Adhesive Powder for Skim Coat/Wall Putty HPMC. Proteins were also determined by Biuret and Kjeldhal'4X4 Galvanized Welded Wire Mesh Panel (FACTORY)(Electrical PV DC Solar Cable). The filtrate in branch B was treated in the same way as branch B inFruit And Vegetable Enzyme Drink. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cotton Yarn Computerized Lace Knitting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Caps Making Machine / Injection Molding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCorrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Safest Baby Healthcare and Grooming Kit for Baby Gift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for SaleandFashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. Enzymatic (Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro)Micro DC 24V Worm Gear Brushless Motor for Medical Equipment(sulfuric acid)1.6 Ton Hand Lifting Tool Lever Chain Block(34*25cm Office File Pocket Felt A4 Document Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Machining Service High Precision CNC Mechanical Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Machining Service High Precision CNC Mechanical Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate) for the financial support.

REFERENCES

Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[cnmirant.com]

Brown, E. M; Thompson, C .J.,China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, M.M.(1994),China PAC Water Flocculating Agent for Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[2ton R Type Electric Wire Rope Hoist with Single Speed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Peb Steel Structure Building Materials H-Section I Beam Steel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Price High Carbon Isostatic Graphite Electrodes. Analytical Biochemistry212,436-445[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Dalev, P.G. and Simeonova, L.S.(1992),Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets. Biotechnology letters 14,6, 531-534[Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor]

Heidemann, E.(1997),Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film.Leder 48,(5),99-104[Coco Amines Supplier & Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Structural Synthetic Fiber for Concrete, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. USP/SP[Accept OEM New Products Team Various Colors Gold Ribbon]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Portable Installation and Ce Certification 20g/Hr OEM Air Purifier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Powder/Spices Powder Packing Machine with Four/Three Side Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coreless Dc Motor 12v]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Black Phosphated Bugle Head Csk Head Drywall Screw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Black Phosphated Bugle Head Csk Head Drywall Screw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[S Series China Generator Helical-Worm Crushers Reduction Box]

Black Phosphated Bugle Head Csk Head Drywall Screw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, Part I.Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pre-Seasoned Cast Iron Baking Stone Cookware]

6 Inch Plastic Breathing Cap for The IBC Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, 175-184[30mm Cutout 1w Mini Downlight]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

Whitaker,J.R.(1963),Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. Analytical Chemistry35(12), 1950-1953[Concrete Spare Part Electrical System Central Distribution Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

No. 1062 20PCS ToolsAuthor for correspondence