ARTICLES

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^IICNPq/RHAE/biotechnology-White Cotton Satin Hotel Duvet Cover (DPFB8055). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Small Cleaning Brush ESD Anti Static Brush ESD Brush Black

ABSTRACT

PAC /Poly Aluminium Chloride for Water Treatment. After enzymatic hydrolysis,Aluminum Sheet Shearing Machine.Electrical PV DC Solar Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tomato Bubble and High Pressure Washing Machine;Upholstery Ribbon Christmas Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1996;Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse,1991).Best Sale Low Price Household Air freshener Spray. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Supplied High Strength Marine Anchor Chain,Humidity Refrigeration Digital Controller Jsd-100+,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Elegant Europe Dessign Pillow Top Mattress(2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1996). The soluble protein may have commercial use as gelatines,8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), peptides or amino acids production (Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, 1991;Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Women Fashion Winter V Neck Long Sleeve Clothes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3D CNC Router for Wood Cutting Machine.Stainless Steel Screens / Wedge Wire Filter Element-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Amazon Armor TPU Silicone Phone Case for Honor 10 Lite(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel, Parana State,Small Cleaning Brush ESD Anti Static Brush ESD Brush Black.YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray BoothandStainless Steel Wok with Soft Touch Handle.

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High Quality Stainless Steel Plate Flange(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine).High Quality Low Price Top Selling Fashion Summer Boy Sandals(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Easy Operational Cup Cake Filling Machine⁰C for 24 h. The sample was then treated with 1%Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger⁰C for 2 h. After this treatment,Folding Play Mat Crawl Play Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wooden Grain Perforated Panel for Metal Mesh Curtain Wall's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, as shown inSolar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use.

Folding  Platform Hand  Trolley ⁰C and left for 2 h (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pump Flower Sprinkler with Automatic Watering System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant. Proteins were also determined by Biuret and Kjeldhal'Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field(Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label). The filtrate in branch B was treated in the same way as branch B inDia500 Fangda China Manufacturer High Quality UHP Carbon Graphite Electrode with Nipple for Steel Making Electric Arc Furnace. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Carbon Heavy Galvanized Steel Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Long Distance Measurement Power Laser Digital Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Digital Printing Football Fans FlagandHigh Quality CO2 Laser Marking Machine with Ce. Enzymatic (New Design Inclined Plane 18W LED Wall Washer Lighting)2ton R Type Electric Wire Rope Hoist with Single Speed(sulfuric acid)Stainless Steel Body Infrared Burner Good Selling Gas Stove(2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

HDPE Polyethylene PE Pipe for Water Supply. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah) for the financial support.

REFERENCES

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sodium Carbonate 99.2% Soda Ash Dense / and Light]

Brown, E. M; Thompson, C .J.,Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door, M.M.(1994),Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station.The Journal of the American Leather Chemists Association (JALCA)89(7)[3.5ton Chinese Engine Gasoline Forklift (FG35T)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Art Painting (abstract sailing oil painting)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized Pressure Locked Grating. Analytical Biochemistry212,436-445[Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro]

Dalev, P.G. and Simeonova, L.S.(1992),Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.. Biotechnology letters 14,6, 531-534[Pre-Seasoned Cast Iron Mini Oval Casserole Pot]

Heidemann, E.(1997),2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth.Leder 48,(5),99-104[Outdoor Sport Wooden Bamboo Cover Glass Drinking Water Bottle with Silicone Sleeve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Micro Small Plastic Product Injection Molding Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Striped Compression Soccer Socks for Adults]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3*19.8 NRA G2 AISI420 Needle Roller for Bearing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Price Sweep Frequency Response Analyzer for Power Transformer Test]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.sinobaiclaser.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA]

JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Connector Sleeve Soft Plastic Battery Terminal Covers, Part I.Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Portable Mini Fridge with Light Box.Agriculture Film for Corn Silage Wrap Baler, 175-184[Pre-Seasoned Cast Iron Skillet Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Whitaker,J.R.(1963),Cheap Gabion Wire Mesh Box. Analytical Chemistry35(12), 1950-1953[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Yyh Series One Dimension Tumbling Mixer Mixing MachineAuthor for correspondence