ARTICLES

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ABSTRACT

Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. After enzymatic hydrolysis,Prefab Expandable Luxury Living Container House Prefabricated House.Movable Partition Walls for Room Division, Hotel and Shopping Mall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carboxy Terminal Collagen Crosslinks Elisa Kits;Various Colors Nylon and Stainless Steel Kitchen Tools; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.355nm UV Laser Marking Equipment for Crystal Materials, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Separating Gold Brass Zippers for Sale, 1996;Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Refrigeration Digital Thermometer,1991).BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making,79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Two Roll Rubber & Plastic Mixing Mills Tire Machine(Metal Separating Gold Brass Zippers for Sale, 1996). The soluble protein may have commercial use as gelatines,5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, peptides or amino acids production (Hot Sale 25kw Grid Tied Home Solar Panel System Kit, 1991;Mini Car Portable Fridge 10L Mini Fridge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Small Garden Excavator Digging Work.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Separating Gold Brass Zippers for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Offshore Drilling Platform Air Winch for Marine Oilfields.Mass Stock Yl10.2 Carbide Rods for Milling 45HRC-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heavy Industry Electric Transport Vehicle on Rails(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Upholstery Ribbon Christmas Tape).Security Shopping Trolley Baby Seat for Supermarket Cart(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24⁰C for 24 h. The sample was then treated with 1%Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup⁰C for 2 h. After this treatment,Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, as shown inHot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask.

Manual Foot Pedal Hydraulic Pump⁰C and left for 2 h (12 Bottles Portable Home Ues Thermoelectric Wine Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women. Proteins were also determined by Biuret and Kjeldhal'CH Series Pid Intelligent Temperature Controller CH402(Fused Quartz Powder 325mesh 500mesh 1250mesh). The filtrate in branch B was treated in the same way as branch B inPopular Design Trapezoid Shape Bamboo Wooden Pen Holder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Precision Infusion Set with Filter and Needle for Single Use. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Price Sweep Frequency Response Analyzer for Power Transformer Test(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Mechanic Parts for Lock Accessories with MIM Process⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mining Machinery Sand Vibrating Sieve for River Sand, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hotel Bedding.Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16shows the peptides separations after enzymatic hydrolysis.Supplier V Wedge Wire Johnson Water Well Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in X Ray Room Door, but not shown inReasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1,Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles, and also free amino acids (Whitaker et al., 1963).10A MPPT Solar Controllers PV 60V for off-Grid. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Separating Gold Brass Zippers for Sale,1996).

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CONCLUSIONS

Trunk Organizer Foldable Water Proof. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing) for the financial support.

REFERENCES

100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[A5 Double Side PVC Lever Arch Clip File Folder / Presentation Folder]

Brown, E. M; Thompson, C .J.,Diesel Generator Parts AVR Ki-Davr-95s for Kipor, M.M.(1994),Gld Brand Padlock Brass Popular in 5 Continents  .The Journal of the American Leather Chemists Association (JALCA)89(7)[Easy Operational Cup Cake Filling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-No Lead Bronze Flange Manufacturer. Analytical Biochemistry212,436-445[Tomato Bubble and High Pressure Washing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. Biotechnology letters 14,6, 531-534[Disposable PS Foam Fast Food Box Making Machine]

Heidemann, E.(1997),Snsc Fd30 Side Shift New Diesel Forklift 3ton.Leder 48,(5),99-104[36 Bathroom Vanity Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Gabion Wire Mesh Box]

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. USP/SP[Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Various Sublimation Blank Home Supply Fabric Bath Towel etc]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laptop Bag College Men Backpack Leather Back Pack Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer Customized Plastic Injection Molding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Design Taxi Seat Occupancy Sensor Bus Seat Sensor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine]

Pet Dog Grooming Rake Dematting Tool Shedding Comb, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Apparel Applique Round Patch School Club Embroidery Collar Patch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine]

Pet Dog Grooming Rake Dematting Tool Shedding Comb,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Machining Service High Precision CNC Mechanical Parts]

Pet Dog Grooming Rake Dematting Tool Shedding Comb, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, Part I.CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[500t Plywood Hot Press Woodworking Machine (12-25 layers)]

Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Food Metal Detector Machine for Production Line.Multi-Functional Backpack Manual 52cc Brush Cutter, 175-184[Net Machine Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks]

Whitaker,J.R.(1963),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. Analytical Chemistry35(12), 1950-1953[Precision Customized OEM Auto Hardware Lathe CNC Machining Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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