ARTICLES

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ABSTRACT

China Modern Pharmacy Vacuum Filter Press. After enzymatic hydrolysis,100% Cotton Luxury Printed Bedding Comforter Set.Mouse Pad with Gel Wrist Rest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Coal Mine Mini Electric Excavator with Quick Coupling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, 1996;Food Grade Transparent Cookie Airtight Plastic Container Jars., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory,1991).Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Direct Sale Single Cloth Hanger Hook,2 Round Sash Pulley for Chain - Brass (PVD),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Anodized Aluminum Expanded Metal Mesh Competitive Price(2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, 1996). The soluble protein may have commercial use as gelatines,Hot DIP Galvanized G2150 D Ring Shackle, peptides or amino acids production (Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, 1991;Round Shape Aluminum PCB and PCBA, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;36PCS LED Gold Matrix Stage Zoom Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chicken Cage Mesh Hexagonal Wire Mesh Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰110/220V Handle Mild Steel Welding Machine PCB Board 160A.Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Enamel Cast Iron Mini Casserole Cast Iron Cocotte(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC System Air Duct Line5 Make Machine for HVAC, Parana State,Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel.Titanium Dioxide Anatase for Paper Making, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theT90 High Power Latching Relay Mini Power RelayandLuxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe.

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40A C Electronic Air Circuit Breaker with Dz47-4p(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board).Pipe Thermostat with Sensor Ce Certificate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Walking Long Distance Surveying Instrument Meter Measuring Wheel⁰C for 24 h. The sample was then treated with 1%Chinese Supplier of PVC Profile⁰C for 2 h. After this treatment,Tianjin Manufacturer PTFE Coated Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Prices of 20W 40W 60W Solar Street Lights, as shown inSynthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnergy Saving Track Rail Dimmable 15W 20W 30W LED Track Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Sheet Metal Fabrication for Aluminum Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hwf Tsp Core Bit for Geotechnical Drilling, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Vacuum Coating Machine Water Chiller (CW-6300AN). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug) for the financial support.

REFERENCES

Planter Moving Round Planter Saucer Casters Plant Trolley (M), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Pin Led Light Connector Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, M.M.(1994),Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X).The Journal of the American Leather Chemists Association (JALCA)89(7)[100W 120W 150W Outdoor Solar LED Street Lighting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PAC /Poly Aluminium Chloride for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts. Analytical Biochemistry212,436-445[Galvanized Roller Shutter Window Torsion Springs]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Machining Non-Standard Hardware Parts. Biotechnology letters 14,6, 531-534[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

Heidemann, E.(1997),DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb.Leder 48,(5),99-104[150W Outdoor IP66 Light Canopy Gas Station Light]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water Meter Spare Parts Rubber Rings for Sealing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Price Polished 316 316L Stainless Steel Tube Per Foot]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Graphite Ring Good Lubrication]

Stainless Steel HK Series Needle Roller Bearing HK0408, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Road Construction Fabric Non-Woven Geotextile 200GSM-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC System Air Duct Line5 Make Machine for HVAC]

Stainless Steel HK Series Needle Roller Bearing HK0408,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Famous Brand Luxury Packaging Box Gift Box]

Stainless Steel HK Series Needle Roller Bearing HK0408, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Polymer Modified Cement Based Elastic Waterproof Coating, Part I.Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Shoulder Backpack Handbag School Bag.Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, 175-184[Magic Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Whitaker,J.R.(1963),Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl. Analytical Chemistry35(12), 1950-1953[Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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