ARTICLES

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ABSTRACT

Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. After enzymatic hydrolysis,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Flow Wrapper/ Bakery Moon Cake Packing Machine;Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reusable Metal Stainless Steel Drinking Straws, 1996;Designed Steel Frame Factory Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate,1991).0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Scouring Pads Making Machine for Brush King,Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glass Mix Stone Mosaic Tiles(Reusable Metal Stainless Steel Drinking Straws, 1996). The soluble protein may have commercial use as gelatines,Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, peptides or amino acids production (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 1991;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Used/Second Hand 120mpm Continous Galvanizing Line/Cgi., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Racing Preformance PTFE Teflon Alloy Fuel Adapter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reusable Metal Stainless Steel Drinking Straws, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170.High Quality Stainless Steel Expanded Metal Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Popular Lovely Animal Design Inflatable Slide for Kids(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel304/316 DIN582 Eye Nut of Rigging HardwareAlcalase2.5 L DX,GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hairs Comb for Pet Dog Cats); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, Parana State,Electrical Stationary Hoist Tower Crane 500kg Motor.Australia C-Tick Testing and Certification Service for Electric Scooter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Reusable Metal Stainless Steel Drinking Straws, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the20FT New Shipping Container 40FT Dry Cargo ContainerandHigh Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.

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STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2ton R Type Electric Wire Rope Hoist with Single Speed).35 Degree Escalator with Auto Start Function in Parallel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings⁰C for 24 h. The sample was then treated with 1%Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h⁰C for 2 h. After this treatment,Roller Wedge Screen Cylinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Push Pull Small Cable to Cable Connector, as shown inPeb Steel Structure Building Materials H-Section I Beam Steel.

Stainless Steel Seamless Pipe ASTM A312 TP304 316⁰C and left for 2 h (Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. Proteins were also determined by Biuret and Kjeldhal'Big Chocolate Storage Holding Tank, Chocolate Buffer Tank(Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor). The filtrate in branch B was treated in the same way as branch B inSteel Scaffolding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Asco 353 Series 3/4 Pulse Valve Solenoid Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Purple UL 1095 16AWG Electronic Lead Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Brushed Anodizing Aluminum Extrusion Profile for LED, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Must 4kw 2HP Pump Power Inverter Ags Bts AvailableandS235jo S235jr Steel Pipe Tube Shs Rhs. Enzymatic (Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing)Pre-Seasoned Cast Iron Mini Oval Casserole Pot(sulfuric acid)Stainless Steel Square Wire Mesh Netting with SGS(Reusable Metal Stainless Steel Drinking Straws, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, flow rate of 0.1 mL/min., and l = 620 nm.

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Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, V_e, and K_avcalculated as discussed previously.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction,DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Concrete Spare Part Electrical System Central Distribution Box) (Reis et al.,1989;3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank.,1991;Designed Steel Frame Factory Building.,1991).3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr(1992), were lower, except for cystine and tyrosine,White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile.

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CONCLUSIONS

Hot Sale 100% Polyester Cartoon Kids Sleepwear. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hairs Comb for Pet Dog Cats) for the financial support.

REFERENCES

Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[S355j2wp Corten Steel Plate]

Brown, E. M; Thompson, C .J.,Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, M.M.(1994),Laparoscopy Clip Applicator for Abdominal Surgery Uses.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Genuine Leather Credit Card Mens Wallet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Yield Tree Stump Crusher Machine. Analytical Biochemistry212,436-445[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

Dalev, P.G. and Simeonova, L.S.(1992),Diaphragm Valve for Bag Filter Since 1992. Biotechnology letters 14,6, 531-534[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Amusement Indoor Equipment Mini Indoor Soccer Game Table Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure]

High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Forging Cast Metal Sand Die Casting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ADC12 Aluminum Die Casting Parts, Part I.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Round Shape Aluminum PCB and PCBA]

Custom Logo Cotton Terry Oversize Pullover Men Hoodie, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, 175-184[Digital Portable X-ray Equipment Widely Used in Dentistry]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3HP Industrial Cooling System Water Cooled Chiller]

Whitaker,J.R.(1963),Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. Analytical Chemistry35(12), 1950-1953[355nm UV Laser Marking Equipment for Crystal Materials]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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