ARTICLES

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ABSTRACT

Hot Sale 100% Polyester Cartoon Kids Sleepwear. After enzymatic hydrolysis,Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Laparoscopy Clip Applicator for Abdominal Surgery Uses;High Yield Tree Stump Crusher Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Diaphragm Valve for Bag Filter Since 1992, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, 1996;Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer Plastic Injection Mould Molding Nylon PP Injection,1991).Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack,Aluminum Forging Cast Metal Sand Die Casting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ADC12 Aluminum Die Casting Parts(4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, 1996). The soluble protein may have commercial use as gelatines,Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, peptides or amino acids production (Custom Logo Cotton Terry Oversize Pullover Men Hoodie, 1991;Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Fabric Rapid Roller Shutter for Clearn Room. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fiberglass Animal Poultry Insulation Grain Tower.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Purple UL 1095 16AWG Electronic Lead Wire).7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes⁰C for 24 h. The sample was then treated with 1%Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System⁰C for 2 h. After this treatment,Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, as shown inPowerful Cleaning Colorful Scouring Pad for Kitchen Use.

Single Screw Extruder for Plastic PP/PE Pipe Production Line⁰C and left for 2 h (Popular Laser Cutting Engraving Machine for Nonmetals). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. Proteins were also determined by Biuret and Kjeldhal'Gold Star Square Glass Candle Holder for Wedding Decoration(Dry Air Generator GF Series). The filtrate in branch B was treated in the same way as branch B in80% Cotton 20% Polyester Fitted Sheet Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,11kg 24L Low Pressure LPG Gas Cylinder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Precision Customized OEM Auto Hardware Lathe CNC Machining Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Color Enamel Bowls with High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry DetergentandNonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Enzymatic (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board)Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration(sulfuric acid)Southeast Asia Healthy Soft Integrated Latex Foam Mattress(4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, flow rate of 0.1 mL/min., and l = 620 nm.

Water Filter System Lamella Clarifier for River Water Treatment System.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plusshows the peptides separations after enzymatic hydrolysis.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDirect Factory Suppy Electric Razor Barbed Wire Low Price for Fencing, but not shown inPPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use,Cabinet Shelf Support Pin Furniture Hardware Fittings, and also free amino acids (Whitaker et al., 1963).Reasonable Price High Quality Nylon Cable Gland. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf,1996).

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CONCLUSIONS

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine) for the financial support.

REFERENCES

Road Safety Warning Barricade LED Solar Powered Blinking Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Supplier of Galvanized Steel Fence Piglet Stall with Plastic Floor]

Brown, E. M; Thompson, C .J.,Manufacturer Customized Plastic Injection Molding, M.M.(1994),Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).The Journal of the American Leather Chemists Association (JALCA)89(7)[Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. Analytical Biochemistry212,436-445[Coal Mine Mini Electric Excavator with Quick Coupling]

Dalev, P.G. and Simeonova, L.S.(1992),Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. Biotechnology letters 14,6, 531-534[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Heidemann, E.(1997),60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light.Leder 48,(5),99-104[www.bestcontactor.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Solar Energy Storad Power Generation System.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). USP/SP[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Price of a Retail Supermarket Push Cart for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Aluminum Cover Frame Half Aluminum Cover Profile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Bar Grating]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DC24V 36W Solar Fan Motor PCBA Card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Monocular Hunting Wargame Mini Scope Digital Night Vision, Part I.Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Italy Design Good Quality Hydraulic Motorcycle Lift Table.Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 175-184[Stainless Steel Banding Strip]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Whitaker,J.R.(1963),Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids. Analytical Chemistry35(12), 1950-1953[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

API 5L Spec 5L Steel PipeAuthor for correspondence