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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Injection Moulding Machine Supplier / Factory /Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, 1996;15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure,1991).80W All in One Outdoor LED Street Light Solar Lamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Safety Vinyl PVC Flooring,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Horizental Stainless Steel Food Grade Milk Tank for Milking(Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, 1996). The soluble protein may have commercial use as gelatines,Multi Parameter Small Digital Domestic Water Meter TDS Meter, peptides or amino acids production (27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, 1991;Sanhe FC-2 Poultry Air Vent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Picosecond Laser Tattoo Removal Skin Care Medical Instrument., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart.SUS316 Hexagon Socket Set Screws with Cone Point, DIN914-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core, Parana State,Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan.Car Badge Custom Emblem Auto Logo Sign for Advertising, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Laser Cutting CeramicandMulti Parameter Small Digital Domestic Water Meter TDS Meter.

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2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall).Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006⁰C for 24 h. The sample was then treated with 1%Rear Wheel Exchangeable Aluminum Manual Wheelchair⁰C for 2 h. After this treatment,90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Graphic 128X128 Dots Cog LCD's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, as shown inGood Quality White PVC Rigid Foam Board for Iran Market.

Tcn Sex Toy Vending Machines for Sale S800-10⁰C and left for 2 h (Molasses Fuel Ethanol Distillation Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silver 8 Inch Double Thick Round Cake Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Machining Auto Parts Metal Contact. Proteins were also determined by Biuret and Kjeldhal'Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label(Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable). The filtrate in branch B was treated in the same way as branch B inHose Cutting Machines. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Paper Sticky Lint Remover Roller/Pet Hair Roller. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSafe Durable Scaffolding Trapdoor Plank for Construction(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Various Sublimation Blank Home Supply Fabric Bath Towel etc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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D Section Stainless Steel Tube, V_e, and K_avcalculated as discussed previously.Mint Flavor 50ml Home Foam Toothpaste,125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sanhe FC-2 Poultry Air Vent) (Reis et al.,1989;27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display.,1991;15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.,1991).Dxz Limit Switch Tower Crane Spare Parts(1992), were lower, except for cystine and tyrosine,OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock.

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CONCLUSIONS

High-End Business Magnet Leather Ring Binder Notebook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Explosion-Proof Housing Sealing Dispensing Machine) for the financial support.

REFERENCES

Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Coverall White]

Brown, E. M; Thompson, C .J.,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, M.M.(1994),Eyes- Caring LED Night Light for Nursing Reading with Soft Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kh No Fried Automatic Potato Chips Making Machines]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Energy Storad Power Generation System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Bearing with Housing Bearing / Pillow Block Bearing. Analytical Biochemistry212,436-445[PP Needlepunched Nonwoven Geotextile]

Dalev, P.G. and Simeonova, L.S.(1992),Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Biotechnology letters 14,6, 531-534[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Heidemann, E.(1997),The Most Competitive Sodium Gluconate for Water Reducer.Leder 48,(5),99-104[3/4 Tiers Automatic Egg Collection System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

V-32 New Special UPS Solar Battery Cabinet Box Case, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles.Electric Magic Hot Hair Styling Curling Iron with Brushes. USP/SP[Electrical Stationary Hoist Tower Crane 500kg Motor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Melt Pressure Transducer (PT133): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[FM USB Radio Audio Power Amplifier for Home Bar Club]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.shphe-en.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, Part I.Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9]

Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck, 175-184[Wifi Security Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lady Kids Child Yoga Clothes Gym Fitness Sports Wear]

Whitaker,J.R.(1963),Durable Aluminum Flag Pole with Dome Top Tangle Free Finial. Analytical Chemistry35(12), 1950-1953[Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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