ARTICLES

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ABSTRACT

6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap. After enzymatic hydrolysis,30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps.Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Forming Pushing Elbow Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 1996;Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (As568 Standard Brown FKM Rubber O Ring for Sealing,1991).The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card,200W UFO LED High Bay Light Fixture for Interior Exterior Applications,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)(13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 1996). The soluble protein may have commercial use as gelatines,13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, peptides or amino acids production (Bathroom False Plastic Board Interior Decorative 3D Wall Panels, 1991;Mushroom Head Stud Sert, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Borosilicate Glass Baking Dish Set Clear. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.Embossing Roller for Tissue Paper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Prefab Steel Building for Garment Factory⁰C and left for 2 h (Mouse Pad with Gel Wrist Rest). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cable Tray with ′t′ Slots. Proteins were also determined by Biuret and Kjeldhal'10 Gauge Galvanized Welded Wire Mesh for Construction Using(European Good Quality Bathroom Simple Sliding Glass Shower Cabina). The filtrate in branch B was treated in the same way as branch B in12A Mink European Remy Virgin 100 Human Hair Bundles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Factory Inline Hockey Skate Puck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foska Good Quality Plastic Stapler Set with Staples, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, V_e, and K_avcalculated as discussed previously.Accept OEM New Products Team Various Colors Gold Ribbon,Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Outdoor Garden Furniture Rattan Sofa Set with Ottomen) (Reis et al.,1989;Bathroom False Plastic Board Interior Decorative 3D Wall Panels.,1991;Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner.,1991).Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh(1992), were lower, except for cystine and tyrosine,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light.

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CONCLUSIONS

Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Concrete Spare Part Electrical System Central Distribution Box) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, M.M.(1994),Home and Hospital Electric Patient Lift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cake/Cookies/Bread Automatic Package and Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. Analytical Biochemistry212,436-445[Mini Car Portable Fridge 10L Mini Fridge]

Dalev, P.G. and Simeonova, L.S.(1992),M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality. Biotechnology letters 14,6, 531-534[Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product]

Heidemann, E.(1997),Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts.Leder 48,(5),99-104[Cement Brick Block Making Machine Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pink French Loop Terry Knit Fabric for Jacket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Human Body Thermometer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Omni Newest Manager Desk Office Smart Office Table Office Staff Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glutamic Acid - Poly-L-Glutamic Acid Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Transparent LED Display P3.91 Glass Wall LED Screen]

Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Greenhouse Fixing Film Profile Lock Channel and Spring Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Sheet Shearing Machine]

Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wood Grain Embossed Film for Flooring]

Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, Part I.Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Tree Shaving Machine]

RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket.Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 175-184[High Pressure]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits]

Whitaker,J.R.(1963),Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52. Analytical Chemistry35(12), 1950-1953[Black Color Hexagon Bolt with High Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Steel & Plastic Drums LiftersAuthor for correspondence