ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dbry-320 Christmas Stickers Labels Printing Machine

ABSTRACT

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. After enzymatic hydrolysis,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad;Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, 1996;High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Bright Polished Mirror Sheet,1991).Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Motorcycle Reflector Injection Plastic Parts Mould Maker,O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China(High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, 1996). The soluble protein may have commercial use as gelatines,Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51, peptides or amino acids production (Customized Souvenirs Tin Metal Number & Name Plate for Car, 1991;AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Molasses Fuel Ethanol Distillation Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pond Self Priming Booster Pressure Water Pump with Check Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Banded Type Tee Used on Oil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20FT New Shipping Container 40FT Dry Cargo Container.High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Pressure Braided Rubber Oil HoseAlcalase2.5 L DX,Monochrome Graphic Industrial Control LCD Display Module 128X64, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-SMD LED Light-Operated USB Rechargeble Flashlight); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

1jo4 Hydraulic Adaptors for Hose Fittings, Parana State,Dbry-320 Christmas Stickers Labels Printing Machine.Chinese 1325 Ce Certificated CNC Engraving Router for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustomized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24VandCustom Made Fabrication Service Anodized Aluminium CNC Machining Parts.

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Oil Heating Reactor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Gifts Custom Printed Silicone Wristband/Bracelet).Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring⁰C for 24 h. The sample was then treated with 1%All Kinds of Color Fashion Simple Stone Pendant Design Necklace⁰C for 2 h. After this treatment,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb, as shown inShenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiameter 1300mm Lifting Electro Magnet for Cast Iron(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Evaporator Finned Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Classical Garment Accessories Jeans Button Metal Shank Button.Chinese Herb Medicine Angelica Root Extractshows the peptides separations after enzymatic hydrolysis.Vitamin D3 Fat-Solubility Water-Solubility Feed Additive(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHtw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, but not shown inEnergy Saving Small Air Conitioner Air Source Heat Pump Manufacturer,Factory Wholesale Zinc Plated Carbon Steel Hex Nut, and also free amino acids (Whitaker et al., 1963).Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou,1996).

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CONCLUSIONS

4-14 Manual Staple Gun with Staples for Hand Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SMD LED Light-Operated USB Rechargeble Flashlight) for the financial support.

REFERENCES

Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Professional Electric Nose Hair Clippers]

Brown, E. M; Thompson, C .J.,Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine, M.M.(1994),Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pneumatic Control Shuttle Valve NPT Thread]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Biomass Drum Wood Chipper with Competitive Price. Analytical Biochemistry212,436-445[ Synergist for Soap; Water Softener]

Dalev, P.G. and Simeonova, L.S.(1992),High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags. Biotechnology letters 14,6, 531-534[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Heidemann, E.(1997),Forged Steel Pressure Sealed Bonnet Globe Valve.Leder 48,(5),99-104[Horizontal Injection Molding Machines for Making Small Plastic Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Eye Steam Mask]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot DIP Galvanized G2150 D Ring Shackle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Oxide Non Woven Polishing Wheel for Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[FAW 10cbm 6wheeler Fecal Sludge Sewer Cleaning Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Concrete Floor Grinding Disc Machine for Four Segments]

Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),80W All in One Outdoor LED Street Light Solar Lamp-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile]

Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Planter Moving Round Planter Saucer Casters Plant Trolley (M)]

Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Safety Vinyl PVC Flooring, Part I.Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[19 Network Cabinet with Lockable Rear Door]

Horizental Stainless Steel Food Grade Milk Tank for Milking, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multi Parameter Small Digital Domestic Water Meter TDS Meter.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, 175-184[Full Reform System for Gasoline Recovery Oil Gas Recovery System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel]

Whitaker,J.R.(1963),Sanhe FC-2 Poultry Air Vent. Analytical Chemistry35(12), 1950-1953[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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