ARTICLES

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ABSTRACT

Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. After enzymatic hydrolysis,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V.Stainless Steel Cheese Making Machine Electric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet and Animal Food Extrusion Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1996;Hexagonal Wire Mesh Chicken Wire Mesh High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (V-32 New Special UPS Solar Battery Cabinet Box Case,1991).4-14 Manual Staple Gun with Staples for Hand Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan,Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness(Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1996). The soluble protein may have commercial use as gelatines,Biomass Drum Wood Chipper with Competitive Price, peptides or amino acids production (High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, 1991;Forged Steel Pressure Sealed Bonnet Globe Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Injection Moulding Machine Supplier / Factory /Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.80W All in One Outdoor LED Street Light Solar Lamp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Safety Vinyl PVC Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sanhe FC-2 Poultry Air Vent, Parana State,Factory Wholesale Zinc Plated Carbon Steel Hex Nut.Picosecond Laser Tattoo Removal Skin Care Medical Instrument, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theManufacturer Customized Plastic Injection MoldingandFull Automatic Tomato Ketchup Sauce Sachet Packaging Machine.

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XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Machining Service High Precision CNC Mechanical Parts).Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart⁰C for 24 h. The sample was then treated with 1%SUS316 Hexagon Socket Set Screws with Cone Point, DIN914⁰C for 2 h. After this treatment,Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, as shown in500t Plywood Hot Press Woodworking Machine (12-25 layers).

Explosion-Proof Housing Sealing Dispensing Machine⁰C and left for 2 h (Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tube Mills for Thin Wall Tubing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. Proteins were also determined by Biuret and Kjeldhal'Car Badge Custom Emblem Auto Logo Sign for Advertising(Precision Customized OEM Auto Hardware Lathe CNC Machining Parts). The filtrate in branch B was treated in the same way as branch B inCutting Machine Xclp3-80 Details. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Graphic 128X128 Dots Cog LCD, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Blue Steel Packing StripandTcn Sex Toy Vending Machines for Sale S800-10. Enzymatic (Silver 8 Inch Double Thick Round Cake Board)High Precision Machining Auto Parts Metal Contact(sulfuric acid)Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label(Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, flow rate of 0.1 mL/min., and l = 620 nm.

Paper Sticky Lint Remover Roller/Pet Hair Roller.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)shows the peptides separations after enzymatic hydrolysis.Safe Durable Scaffolding Trapdoor Plank for Construction(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10A MPPT Solar Controllers PV 60V for off-Grid, but not shown inFTTH Fiber Optic Fusion Splicing Toolkit,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, and also free amino acids (Whitaker et al., 1963).Various Sublimation Blank Home Supply Fabric Bath Towel etc. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer,1996).

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Best Quality Bending Metal Mould with All Kinds Metal Materials, V_e, and K_avcalculated as discussed previously.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus,Milling Precision Stainless Steel CNC Machinery Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line) (Reis et al.,1989;High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags.,1991;Hexagonal Wire Mesh Chicken Wire Mesh High Quality.,1991).U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails(1992), were lower, except for cystine and tyrosine,Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.

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CONCLUSIONS

Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi Parameter Small Digital Domestic Water Meter TDS Meter) for the financial support.

REFERENCES

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[800mm UV Putty Coating Filling Machine for Solid Wood/MDF/Veneer/Ply/Fiber Cement/Silicate Board]

Brown, E. M; Thompson, C .J.,360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), M.M.(1994),Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW).The Journal of the American Leather Chemists Association (JALCA)89(7)[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Machine Cube Ice Machine for Commercial Use. Analytical Biochemistry212,436-445[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

Dalev, P.G. and Simeonova, L.S.(1992),Christmas Decoration Rose Flower LED Light String. Biotechnology letters 14,6, 531-534[Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge]

Heidemann, E.(1997),D Section Stainless Steel Tube.Leder 48,(5),99-104[Featured]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)]

Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mint Flavor 50ml Home Foam Toothpaste.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. USP/SP[As568 Standard Brown FKM Rubber O Ring for Sealing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Roll Plastic T-Shirt Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dxz Limit Switch Tower Crane Spare Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Density White Magic Sponge Magic Eraser Melamine Foam Cleaning Sponge Car Cleaning Sponge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag]

OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High-End Business Magnet Leather Ring Binder Notebook-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hotel Steel Digital Room Safes with Large LED Display]

OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder]

OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, Part I.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Logo Cotton Terry Oversize Pullover Men Hoodie]

Eyes- Caring LED Night Light for Nursing Reading with Soft Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Bearing with Housing Bearing / Pillow Block Bearing.Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, 175-184[www.mybarberchairs.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing]

Whitaker,J.R.(1963),The Most Competitive Sodium Gluconate for Water Reducer. Analytical Chemistry35(12), 1950-1953[Custom Aluminum Cover Frame Half Aluminum Cover Profile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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