ARTICLES

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ABSTRACT

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile. After enzymatic hydrolysis,Hot Sale 100% Polyester Cartoon Kids Sleepwear.Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater;Laparoscopy Clip Applicator for Abdominal Surgery Uses; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Yield Tree Stump Crusher Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diaphragm Valve for Bag Filter Since 1992, 1996;4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine,1991).Manufacturer Plastic Injection Mould Molding Nylon PP Injection. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Forging Cast Metal Sand Die Casting(Diaphragm Valve for Bag Filter Since 1992, 1996). The soluble protein may have commercial use as gelatines,ADC12 Aluminum Die Casting Parts, peptides or amino acids production (Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, 1991;Custom Logo Cotton Terry Oversize Pullover Men Hoodie, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diaphragm Valve for Bag Filter Since 1992, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Fabric Rapid Roller Shutter for Clearn Room.Fiberglass Animal Poultry Insulation Grain Tower-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Slewing Gear for Tower Crane Slewing Winch Reducer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Painting Canvas).Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side⁰C for 24 h. The sample was then treated with 1%High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes⁰C for 2 h. After this treatment,Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, as shown inHigh Quality Industrial Grade Anhydrous Sodium Sulfate.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%11kg 24L Low Pressure LPG Gas Cylinder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Use Click Lock Standard Spc Vinyl Plank Flooring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Color Enamel Bowls with High Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Low Carbon Heavy Galvanized Steel WireandCustomized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Enzymatic (Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line)Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board(sulfuric acid)Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration(Diaphragm Valve for Bag Filter Since 1992, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Southeast Asia Healthy Soft Integrated Latex Foam Mattress, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Southeast Asia Healthy Soft Integrated Latex Foam Mattress, flow rate of 0.1 mL/min., and l = 620 nm.

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.50Hz to 60Hz Frequency Inverter for Water Pumpshows the peptides separations after enzymatic hydrolysis.Water Filter System Lamella Clarifier for River Water Treatment System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEmbossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, but not shown inGood Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber,Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), and also free amino acids (Whitaker et al., 1963).Cabinet Shelf Support Pin Furniture Hardware Fittings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diaphragm Valve for Bag Filter Since 1992,1996).

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High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving MachineandStructural Synthetic Fiber for Concreteshow the gel chromatographic separation of soluble protein with Sephadex G-Reasonable Price High Quality Nylon Cable Gland.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, and another peak at 45 mL not shown inBrand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container, for peptides lower than 3000 Da.Disposable Anesthesia Breathing Circuit Tube(Condell et al.,1993) .

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High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, V_e, and K_avcalculated as discussed previously.Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/) (Reis et al.,1989;Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale.,1991;4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf.,1991).Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks(1992), were lower, except for cystine and tyrosine,Counter-Clip Fluorine-Lined Butterfly Valve.

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CONCLUSIONS

DC Brushless Motor for CNC Machine with ISO9001. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle) for the financial support.

REFERENCES

Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6 in 1 Tripolar RF Bio Ultrasonic Cool Oxygen Jet Microdermabrasion Water Dermabrasion Machine]

Brown, E. M; Thompson, C .J.,Road Safety Warning Barricade LED Solar Powered Blinking Light, M.M.(1994),Manufacturer Customized Plastic Injection Molding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lishui Factory High Quality Metal Rolling Ball Screw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08). Analytical Biochemistry212,436-445[Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling]

Dalev, P.G. and Simeonova, L.S.(1992),Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. Biotechnology letters 14,6, 531-534[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Heidemann, E.(1997),Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.Leder 48,(5),99-104[3 Bbl Jacketed Brite Tank Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing]

ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light.Solar Energy Storad Power Generation System. USP/SP[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[60ml 100ml Customized Glass Jar for Spice]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Structure Building In Malaysia]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Humidity Refrigeration Digital Controller Jsd-100+]

Custom Aluminum Cover Frame Half Aluminum Cover Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)]

Custom Aluminum Cover Frame Half Aluminum Cover Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nylon Weatherproof PA6 Strain Relief Cable Gland]

Custom Aluminum Cover Frame Half Aluminum Cover Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DC24V 36W Solar Fan Motor PCBA Card, Part I.New Monocular Hunting Wargame Mini Scope Digital Night Vision. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Bright Polished Mirror Sheet]

Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.Italy Design Good Quality Hydraulic Motorcycle Lift Table, 175-184[Mini Personal Waterproof GPS Tracker with Long Working Days (Y21)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core]

Whitaker,J.R.(1963),Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. Analytical Chemistry35(12), 1950-1953[Electrical Barber Shop Equipment Electric Hair Curling Wand Iron]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

HDPE Polyethylene PE Pipe for Water SupplyAuthor for correspondence