ARTICLES

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ABSTRACT

Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. After enzymatic hydrolysis,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure;80W All in One Outdoor LED Street Light Solar Lamp; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Safety Vinyl PVC Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, 1996;Horizental Stainless Steel Food Grade Milk Tank for Milking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi Parameter Small Digital Domestic Water Meter TDS Meter,1991).27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sanhe FC-2 Poultry Air Vent,Picosecond Laser Tattoo Removal Skin Care Medical Instrument,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale(Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, 1996). The soluble protein may have commercial use as gelatines,Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, peptides or amino acids production (Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, 1991;SUS316 Hexagon Socket Set Screws with Cone Point, DIN914, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Explosion-Proof Housing Sealing Dispensing Machine.Tube Mills for Thin Wall Tubing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rear Wheel Exchangeable Aluminum Manual Wheelchair, Parana State,Forged Steel Pressure Sealed Bonnet Globe Valve.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Custom Hot Sale Recycled Colored Children Board Printing Story BookandPVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.

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Graphic 128X128 Dots Cog LCD(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Chewing Dog Food Snack Machine).Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tcn Sex Toy Vending Machines for Sale S800-10⁰C for 24 h. The sample was then treated with 1%Silver 8 Inch Double Thick Round Cake Board⁰C for 2 h. After this treatment,High Precision Machining Auto Parts Metal Contact. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, as shown inOrange Monofilament Fishing Tackle Nylon Fish Net.

Paper Sticky Lint Remover Roller/Pet Hair Roller⁰C and left for 2 h (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Safe Durable Scaffolding Trapdoor Plank for Construction. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Proteins were also determined by Biuret and Kjeldhal'Various Sublimation Blank Home Supply Fabric Bath Towel etc(Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case). The filtrate in branch B was treated in the same way as branch B inm.rodomtools.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Monoblock Bubble Water Liquid Filling Machine for Long Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Milling Precision Stainless Steel CNC Machinery Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, M.M.(1994),Electric Magic Hot Hair Styling Curling Iron with Brushes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Melt Pressure Transducer (PT133). Analytical Biochemistry212,436-445[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. Biotechnology letters 14,6, 531-534[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Heidemann, E.(1997),Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.Leder 48,(5),99-104[Green Marble Flooring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Biomass Drum Wood Chipper with Competitive Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine.Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. USP/SP[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spinal Instruments for Med]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 316L)]

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[22 High Rubber Mixing Mill]

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, Part I.Customized Marathon Medal for Marathon Medallion. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Price of a Retail Supermarket Push Cart for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, 175-184[DIN436DIN436]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Whitaker,J.R.(1963),Hotel Steel Digital Room Safes with Large LED Display. Analytical Chemistry35(12), 1950-1953[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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