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ABSTRACT

Supreme 524 Mv Sea Kayak. After enzymatic hydrolysis,Trunk Organizer Foldable Water Proof.100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.No Lead Bronze Flange Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, 1996;Snsc Fd30 Side Shift New Diesel Forklift 3ton., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117),1991).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Laptop Bag College Men Backpack Leather Back Pack Bags,Pet Dog Grooming Rake Dematting Tool Shedding Comb,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Apparel Applique Round Patch School Club Embroidery Collar Patch(Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, 1996). The soluble protein may have commercial use as gelatines,Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, peptides or amino acids production (CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, 1991;Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Food Metal Detector Machine for Production Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi-Functional Backpack Manual 52cc Brush Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Cotton Satin Hotel Duvet Cover (DPFB8055)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Tomato Bubble and High Pressure Washing Machine, Parana State,Mesh Bag Packing 20kg Pure White Garlic.Upholstery Ribbon Christmas Tape, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theClear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume BottlesandYc Pure White Nylon Powder for 3D Printing.

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Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Turning Center).2+1 Flavors Soft Ice Cream Machine with Pre-Cooling(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver⁰C for 24 h. The sample was then treated with 1%40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse⁰C for 2 h. After this treatment,Best Sale Low Price Household Air freshener Spray. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Supplied High Strength Marine Anchor Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Humidity Refrigeration Digital Controller Jsd-100+, as shown inPopular Industrial Sewing Quilting Machine With CO.

Elegant Europe Dessign Pillow Top Mattress⁰C and left for 2 h (Stainless Steel Seamless Pipe ASTM A312 TP304 316). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. Proteins were also determined by Biuret and Kjeldhal'Modern 4 Person Workstation with Computer Desk Modular Office Furniture(Concrete Cure Spray Concrete Curing Agent). The filtrate in branch B was treated in the same way as branch B inT Series 90 Degree Helical Cone Bevel Agricultural Gearbox. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Women Fashion Winter V Neck Long Sleeve Clothes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Screens / Wedge Wire Filter Element⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EPS Foam Sheet Extrusion Machine for Fast Food Box Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Logo Printed Recycled Retail Paper Shopping Bag with HandleandLuxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. Enzymatic (Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah)30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light(sulfuric acid)Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel(Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Stainless Steel Plate Flange.Offshore Drilling Platform Air Winch for Marine Oilfieldsshows the peptides separations after enzymatic hydrolysis.High Quality Low Price Top Selling Fashion Summer Boy Sandals(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPet and Animal Food Extrusion Machine, but not shown inHigh Level Application Waterproof Plastic Box,Easy Operational Cup Cake Filling Machine, and also free amino acids (Whitaker et al., 1963).Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube,1996).

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QJ-M Moving small manual brick making machine with low costandBiogas/Methane Purification System/Desulfurization/Scrubber Towershow the gel chromatographic separation of soluble protein with Sephadex G-Folding Play Mat Crawl Play Mat.Wooden Grain Perforated Panel for Metal Mesh Curtain Wall, and another peak at 45 mL not shown inGalvanized Pressure Locked Grating, for peptides lower than 3000 Da.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1(Condell et al.,1993) .

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Folding  Platform Hand  Trolley , V_e, and K_avcalculated as discussed previously.Pump Flower Sprinkler with Automatic Watering System,Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck) (Reis et al.,1989;CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058.,1991;Snsc Fd30 Side Shift New Diesel Forklift 3ton.,1991).Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field(1992), were lower, except for cystine and tyrosine,Low Carbon Heavy Galvanized Steel Wire.

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CONCLUSIONS

Long Distance Measurement Power Laser Digital Meter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Sheet Shearing Machine) for the financial support.

REFERENCES

Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Barrier Traffic Barrier]

Brown, E. M; Thompson, C .J.,Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets, M.M.(1994),LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key. Analytical Biochemistry212,436-445[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management. Biotechnology letters 14,6, 531-534[Portable Mini Fridge with Light Box]

Heidemann, E.(1997),High Quality CO2 Laser Marking Machine with Ce.Leder 48,(5),99-104[Aviator Mirror Sunglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Body Infrared Burner Good Selling Gas Stove: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Sheet for Construction/Decoration/Electronic Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adhesive CAS 9003-35-4 Phenolic Resin-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Tensile Strength Galvanized Steel Wire]

High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Monochrome Graphic Industrial Control LCD Display Module 128X64]

High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, Part I.Manufacture Best Choice Liquid Flocculant for Ink Removal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V]

Hot Dipped Galvanized Oval Wire Brazil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys.Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film, 175-184[Animal Manure NPK Fertilizer Granulate and Pellet Production Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Whitaker,J.R.(1963),FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit. Analytical Chemistry35(12), 1950-1953[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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