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ABSTRACT

Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. After enzymatic hydrolysis,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.Water Slide for Indoor Play Structure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder;1220*2440mm Concrete Formwork black film faced plywood; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Washami Teeth Care for Whitening Cool Mint Toothpaste, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1996;Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SHXJ1500 CNC Diamond Wire Stone Cutting Machine,1991).Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine,Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yyh Series One Dimension Tumbling Mixer Mixing Machine(Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1996). The soluble protein may have commercial use as gelatines,ODU 14 Pin Industrial Circular Connector, peptides or amino acids production (Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, 1991;Classical New Coming Plastic Female Sunglasses, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Double Sided Rigid PCB Circuit Board Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Robot of World Robotics Competition.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Rope/Cable/Wire Braiding Machine with Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum PCB Circuit Board SMD PCBA LED PCB.Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

China Supplier Steel Machine Bellows Cover Telescopic Shield CoverAlcalase2.5 L DX,Concrete Cure Spray Concrete Curing Agent, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Automatic Chewing Dog Food Snack Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)White Ceramic Shot Glass for Sublimation Printing - 1.5oz(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Promotional Gifts Custom Printed Silicone Wristband/Bracelet, Parana State,High Level Application Waterproof Plastic Box.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Professional LED Display Ceramic Hair Curling Ironand2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment.

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8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Personal Care Glutathione Skin Whitening Tablets).Farrowing Crate Pig Floor Cast Iron Slat Floor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing⁰C for 24 h. The sample was then treated with 1%High Quality Aluminum Heat Sink for Electronics Computer Electric⁰C for 2 h. After this treatment,5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Surprise! Range Hood Free! ! ! Juice Cart's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steam Eye Mask Heating One Eye Mask for Sleeping, as shown inLd-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant.

Automatic French Fries Frying Machine with Low Price⁰C and left for 2 h (Manufacture Best Choice Liquid Flocculant for Ink Removal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. Proteins were also determined by Biuret and Kjeldhal'Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security(5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type). The filtrate in branch B was treated in the same way as branch B inPure Material HDPE Pipe for Water Supply and Drains. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polyglycolic Acid Absorable Suture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter DoorandNo. 1062 20PCS Tools. Enzymatic (Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant)Aluminum Mould Shoe Mould Making CNC Milling Router Machine(sulfuric acid)High Pressure Fitting Quick Coupling Hydraulic Plug(Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, flow rate of 0.1 mL/min., and l = 620 nm.

Refrigeration Digital Temperature Controller Stc-100A.Professional Rope/Cable/Wire Braiding Machine with Factory Priceshows the peptides separations after enzymatic hydrolysis.Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women, but not shown inCpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts,Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, and also free amino acids (Whitaker et al., 1963).Black Color Hexagon Bolt with High Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope,1996).

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CONCLUSIONS

Single Stage High Speed Fruit and Vegetable Pulping Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Chewing Dog Food Snack Machine) for the financial support.

REFERENCES

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Parts Spark Plugs]

Brown, E. M; Thompson, C .J.,Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, M.M.(1994),Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads.The Journal of the American Leather Chemists Association (JALCA)89(7)[2.0mm Yellow Markers for Fiber Optic Multi Patch Cord]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stand Hot and Cold Normal Water Dispenser with Refrigerator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. Analytical Biochemistry212,436-445[ACP Aluminium Cmposite Panel Cladding Extrusions]

Dalev, P.G. and Simeonova, L.S.(1992),Prefab Expandable Luxury Living Container House Prefabricated House. Biotechnology letters 14,6, 531-534[Hotel Bedding]

Heidemann, E.(1997),Movable Partition Walls for Room Division, Hotel and Shopping Mall.Leder 48,(5),99-104[Drink Bottle Good Smoothie Blender]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)]

Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Carboxy Terminal Collagen Crosslinks Elisa Kits.Various Colors Nylon and Stainless Steel Kitchen Tools. USP/SP[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-355nm UV Laser Marking Equipment for Crystal Materials: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pinch Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Metal Separating Gold Brass Zippers for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Leak Proof]

Metal Separating Gold Brass Zippers for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Metal Separating Gold Brass Zippers for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Refrigeration Digital Thermometer, Part I.BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.Two Roll Rubber & Plastic Mixing Mills Tire Machine, 175-184[Abs Head Foot Board For Hospital Bed]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Whitaker,J.R.(1963),5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. Analytical Chemistry35(12), 1950-1953[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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