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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sofa Materials PU Leather with Flock Backing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Three Phase 800mm Plate Fan

ABSTRACT

ASTM Approved Popular Indoor Trampoline of Park. After enzymatic hydrolysis,Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building.Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Genuine Leather Credit Card Mens Wallet;Yitai Die Making 13mm Beech Rotary Plywood; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gear Rolling Machine Zh28/25 Spline Hobbing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, 1996;Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Partial Thread Steel/Iron Hex Head Self Tapping Screw,1991).Flat Car Sun Roof Tent Awning. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Classic Black Color Commercial Glass Door Multi Deck Refrigerator,Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)(OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, 1996). The soluble protein may have commercial use as gelatines,Pneumatic Control Shuttle Valve NPT Thread, peptides or amino acids production (Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, 1991;OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Styles Fashion Erotic Women Underwear Sexy Lingerie., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stand Hot and Cold Normal Water Dispenser with Refrigerator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Construction Welding Rods Electrode E6013 E7018 E7016.High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pagoda Gazebo Tent in Guangzhou for Sale).14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad⁰C for 24 h. The sample was then treated with 1%Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800⁰C for 2 h. After this treatment,Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, as shown inWaterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).

Aluminum Bright Polished Mirror Sheet⁰C and left for 2 h (Stainless Steel Mechanic Parts for Lock Accessories with MIM Process). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Motorcycle Reflector Injection Plastic Parts Mould Maker. Proteins were also determined by Biuret and Kjeldhal'O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W(High Quality MPO Singlemode Duplex Fiber Optic Adapter). The filtrate in branch B was treated in the same way as branch B inPrivate Label Best Skin Care Moisturizing 24K Gold Collagen Hand Mask. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Souvenirs Tin Metal Number & Name Plate for Car(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Molasses Fuel Ethanol Distillation Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pond Self Priming Booster Pressure Water Pump with Check Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Banded Type Tee Used on Oil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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SMD LED Light-Operated USB Rechargeble Flashlight.China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipesshows the peptides separations after enzymatic hydrolysis.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHanwha SMT Chip Shooter LED Pick and Place Machine, but not shown inTop Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine,1jo4 Hydraulic Adaptors for Hose Fittings, and also free amino acids (Whitaker et al., 1963).Chinese 1325 Ce Certificated CNC Engraving Router for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts,1996).

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CONCLUSIONS

Toilet Roll Tissue Wrapping Machine with Auto - Counting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized 3D Medal for Religious Medal Gift) for the financial support.

REFERENCES

Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[220 Grit Aluminum Oxide Abrasive Brown Fused Sand for Refractory]

Brown, E. M; Thompson, C .J.,Orange Monofilament Fishing Tackle Nylon Fish Net, M.M.(1994),Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002).The Journal of the American Leather Chemists Association (JALCA)89(7)[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Analytical Biochemistry212,436-445[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Dalev, P.G. and Simeonova, L.S.(1992),Diameter 1300mm Lifting Electro Magnet for Cast Iron. Biotechnology letters 14,6, 531-534[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Heidemann, E.(1997),High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%.Leder 48,(5),99-104[Greatshoe New Fashion Design TPR Sole Girl Shoes Sport Running Kids Casual Walking School Children Shoes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gym Fitness Equipment Adjustable Weight Bench]

3kw 24V 310mm Vertical Agv Drive Wheel Assembly, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Evaporator Finned Tube.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear. USP/SP[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reusable Metal Stainless Steel Drinking Straws]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2018 Hot Selling Efficient nonwoven T-Shirt Bag Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

100%Polyester Spandex Hotel Chair Cover (DPH7022), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Perforated Metal Mesh-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ D1400]

100%Polyester Spandex Hotel Chair Cover (DPH7022),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

100%Polyester Spandex Hotel Chair Cover (DPH7022), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Turning Center, CNC Horizontal Turning Milling Machine, Part I.Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure Fitting Quick Coupling Hydraulic Plug]

Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Classical Garment Accessories Jeans Button Metal Shank Button.Vitamin D3 Fat-Solubility Water-Solubility Feed Additive, 175-184[Hex Cap Screw Dimensions]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

Whitaker,J.R.(1963),Factory Wholesale Zinc Plated Carbon Steel Hex Nut. Analytical Chemistry35(12), 1950-1953[ Hv / Coating Material Grade CMC Medium Viscosity]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Efficiency Fine Powder Vibrating ScreenerAuthor for correspondence